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TriFECTaTM_RNAi_User_Guide.pdf(210.9K)  [34]   2010-07-20 10:55:04

Transfections per 1 nmole duplex RNA.

Plate Format

Media Volume (¥ìL)

# of Transfections at 1 nM

# of Transfections at 10 nM

6 well

2500

400

40

12 well

1200

820

82

24 well

600

1660

166

48 well

250

4000

400

96 well

150

6660

666

 

 

 

 

 

 

 

 

 

 

 

* IDT TriFECta ±¸¼º

Kit Contents:

 

The TriFECTa Kit includes 6 tubes that contain RNA duplexes (dry) and 1 tube that contains 1x buffer. All duplexes have been HPLC purified and QC tested by ESI-MS.

 

• Three target-specific Dicer-Substrate siRNA duplexes (2 nmoles each)

• Cy3 TM DS Transfection Control, fluorescent-labeled transfection control duplex (1 nmole)

• HPRT-S1 DS Positive Control duplex (1 nmole)

• DS Scrambled-Neg, universal negative control duplex (1 nmole)

• Rnase-free Duplex Buffer (100mM KAc/30 mM HEPES pH 7.5) (2 mL)

 

 

 


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