Resuspension
•Centrifuge the tube prior to opening to ensure that the DNA is at the bottom of the tube.
•Resuspend DNA in 20 µL of 10 mM Tris, 0.1 mM EDTA buffer (pH 7.5 – 8.0) to reach an approximate concentration of 0.1 µg/µL for a stock concentration.
•Incubate the tube at room temperature for 30 minutes and then vortex for 20 seconds
•Centrifuge for 1 minute
•To create a working concentration, add 1 µL of the stock concentration to 999 µL of water to reach an approximate working concentration of 0.1 ng/µL. Use 1-2 µL of the working dilution for bacterial transformations.
Storage Conditions
Store hydrated DNA at -20°C.
Lyophilized DNA is stable at room temperature for 3 months. We recommend storing at -20°C for longer periods of time.
IDT