PrimeTime Pre-designed qPCR Assays
IDT¿¡¼ Pre-designed qPCR Assays¸¦ Ãâ½ÃÇß½À´Ï´Ù. ±âÁ¸ PrimeTime qPCR Assay´Â NCBI accession number¸¦ ÀÌ¿ëÇØ Scitool¿¡¼ µðÀÚÀÎµÈ sequences¸¦ Á¦°øÇØ ¿Ô½À´Ï´Ù.
ÀÌÁ¦´Â ´õ Áøº¸µÈ Pre-designed qPCR Assay¸¦ ÆǸÅÇÕ´Ï´Ù. IDTÀÇ Ç³ºÎÇÑ °æÇè°ú ±â¼ú·ÂÀ» ¹ÙÅÁÀ¸·Î NCBIÀÇ ÃֽŠ¾÷µ¥ÀÌÆ® sequence¸¦ ÀÌ¿ëÇÏ¿© ±¸ÃàµÈ dzºÎÇÑ library¸¦ ÀÌ¿ëÇÏ¿© ¿Ïº®ÇÑ ½ÇÇèÀ» ±¸ÇöÇϼ¼¿ä. -> ÁÖ¹® ¹Ù·Î°¡±â Ŭ¸¯!!!
[ÁÖ¹® ¹æ¹ý] 1. NCBI accession number ȤÀº gene symbol(±âÈ£, - ¾øÀÌ)À» ÀÔ·Â ÈÄ submit click 2. ¿øÇÏ´Â species³ª exon À§Ä¡ µîÀ¸·Î target ¼±Åà ¹üÀ§¸¦ ÁÙÀδÙ. 3. ¼±ÅÃµÈ targetÀÇ reporter Çü±¤ ¹× »çÀÌÁ °áÁ¤ ÇÑ µÚ order click -> ÁÖ¹® ¿Ï·á
* ¹ßÁÖ ÁøÇà Áß ºÒÆíÇÑ Á¡ÀÌ ÀÖÀ¸½Ã¸é ¾ðÁ¦µçÁö idt@mbiotech.co.kr·Î ¿¬¶ôÁÖ¼¼¿ä Àüȹ®ÀÇ´Â 031-556-3905 ÀÔ´Ï´Ù. Assay ID¸¸ ãÀ¸½Ã¸é ÀüÈ ¶Ç´Â ¸ÞÀÏ·Î ÀúÈñ°¡ ¹ßÁÖ¸¦ ÁøÇàÇØ µå¸®°Ú½À´Ï´Ù. Ư¡: ¡×Pre-designed assays are available for human, mouse and rat
¡×Designed to span every exon boundary in the transcriptome
¡×Current coverage rate for human is 97.4% of exon junctions
¡×Assays are designed to
¡×Avoid SNPs locations
¡×Cross reactivity check to make sure other transcripts are not detected
( eliminate nonspecific amplification) ¡×Reduce impact from secondary structure formation
¡×Uses up to date Refseq info
¡×Maps all the SNP regions and avoids them
¡×Inbuilt Blast search to check for cross reactivity
¡× Splice common and splice specific designs are available
¡×Designing is not live, designs have additional screening criteria incorporated
¡×Can design using NM, NR, XM, and XR
( may change with Ref seq release) * IDT ȨÆäÀÌÁö¸¦ ÀÌ¿ë ½Ã¿¡´Â ¿µ¹®À¸·Î ȸ¿ø°¡ÀÔÀ» ÇÏ¼Å¾ß ÇÕ´Ï´Ù. * IDT ȨÆäÀÌÁö ȸ¿ø °¡ÀÔÀÌ ºÒÆíÇÏ½Ç ¶§¿¡´Â idt@mbiotech.co.kr·Î ¼±ÅÃµÈ Á¦Ç°ÀÇ Assay ID ¹øÈ£¸¦ Áֽøé ÀúÈñ°¡ ´ë½Å ¹ßÁÖ¸¦ ÁøÇàÇØ µå¸³´Ï´Ù.
¡×Cannot design with sequence
-> sequence·Î µðÀÚÀÎÀ» ¿øÇÒ ¶§´Â ±âÁ¸ÀÇ Primetime Assay¸¦ ÀÌ¿ë
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