ÀÌ »ç¿ëÈıâÀÇ Á¦Ç° [ M-MLV Reverse Transcriptase ] º¸±â
¾È³çÇϼ¼¿ä
¼¿ï´ëÇб³ ÈÇлý¹°°øÇкΠ»ý¹°ºÐÀÚ°øÇÐ ½ÇÇè½Ç ÀÔ´Ï´Ù.
ÀúÈñ´Â Real-Time PCRÀ» »ç¿ëÇÏ¿© mRNA expression levelÀ» ÃøÁ¤ÇÏ´Â ½ÇÇèÀ» ¸¹ÀÌ ÁøÇàÇÏ°í ÀÖ½À´Ï´Ù.
±×·±µ¥ ±âÁ¸ÀÇ RTase´Â ºñ½Î¹Ç·Î ½Î¸é¼ È¿À²ÁÁÀº product¸¦ ã´Ù°¡ ¿¥¹ÙÀÌ¿ÀÅØÀÇ M-MLV reverse Transcriptase¸¦ »ç¿ëÇÏ°Ô µÇ¾ú½À´Ï´Ù. °¡°Ý´ëºñ ¸¸Á·µµ´Â ³Ê¹« ±¦Âú½À´Ï´Ù. °¡°Ýµµ °¡°ÝÀÌÁö¸¸ È¿À²µµ ¸¸Á·½º·¯¿ö¼
´Ù¸¥ ½ÇÇè½Ç¿¡µµ °¡²û È«º¸µµ ÇØÁÖ°í ±×·´´Ï´Ù. ÀÌ Á¦Ç° °¡°Ý ¿Ã¸®Áö ¸»¾ÆÁÖ¼¼¿ä~
±×¸®°í IDT»çÀÇ Oligo-dT(15mer)¿Í dNTP(10 mM)ÀÇ °æ¿ìµµ Ä÷¸®Æ¼°¡ ¸Å¿ì ÁÁ¾Ò½À´Ï´Ù.
¾ÕÀ¸·Îµµ ÁÁÀº Á¦Ç° ºÎŹµå¸³´Ï´Ù.
|