PrimeTime qPCR Primer Assays (SYBR)

PrimeTime qPCR Primers (SYBR Green ̿ qPCR)

SYBR Green qPCR Pre-designed Primers
Probe qPCR Ǹŵǰ ִ Primetime qPCR Assays probe ϰ Primer pair tube ־ մϴ.
(500 reactions)
NCBI accession numberε human, mouse, rat transcriptomes pre-design ֽϴ. PrimeTime qPCR Assays Primer pair Ʃ꿡 3-4 ȿ ǰ ֽϴ. ø mass pectrometry 100 % QCģ Ŀ ǰ ǸŵǸ QC data IDT Ȩ ٿε մϴ.

Primetime qPCR SYBR assay Ư¡

••100 % QC oligos
••Full sequences
••90 %
••Human, mouse, rat
••Primer pair tube Բ
••3-4 ҿ

PrimeTime qPCR Primer Assays

No. of Reactions (20 L)
Estimated Ship Date Quantity (nmoles)
PrimeTime qPCR Primers


Pre-designed PrimeTime qPCR Assay

* 100 % (positive negative control )
* Sequence (ǰ )
* Human, mouse, rat species

Pre-designed PrimeTime qPCR Assay

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Same High Efficiency With or Without Probe 
Panel A. Amplification with SYBR Green
Panel B. Amplification with Dual-Labeled Probe.
Figure 1. PrimeTime Assays Yield the Same High Efficiency Whether Used With Intercalating Dyes or Probe. Amplification of 5 sequential 4-fold dilutions of cDNA using PrimeTime qPCR Primer Assays (with SYBR Green dye) or the PrimeTime qPCR 5' Nuclease Assay (with dual-labeled probe) to human 3-oxoacid CoA transferase 1 (OXCT1) (NM_000436).
Figure 2. PrimeTime qPCR Primer Assays Have Average Reaction Efficiency >90%. Sixty randomly selected PrimeTime qPCR Primer Assays and 15 PrimeTime qPCR Primer Assays for endogenous control genes used with Brilliant III Ultra Fast SYBR Green qPCR Master Mix (Agilent) were analyzed over 5 sequential 4-fold dilutions (50–0.195 ng/reaction) of cDNA prepared from Universal Human Reference RNA (Agilent). Reactions were run on the 7900HT Fast Real-Time PCR System (Applied Biosystems) using PCR cycling conditions: 3 min 95C; 45 x (5 sec 95C, 15 sec 60C). Average reaction efficiencies for the assays tested exceeded 98%.
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Custom DNA Oligos
SensiFAST SYBR Hi-ROX One-Step Kit