OMNIfect¢â transfection reagent is an easy-to-use, cationic delivery reagent providing high efficiency transfection of plasmid DNA coupled with low cytotoxicity across a broad range of cells. OMNIfect has been tested across a variety of cell lines and has proven to successfully transfect both adherent and suspension cell lines.
Figure 1: HEK-293, COS-1, HepG2, C2C12, CHO and Jurkat cells were seeded in 12-well plates and transfected with 1µg luciferase plasmid according to the manufacture¡¯s protocols. At 48 hours after transfection luciferase expression was quantified (relative light units /mg total protein) from cell lysates. Data are presented as values (+/- SEM) relative to OMNIfect transfection. High transfection efficiencyOMNIfect Transfection Reagent has been proven to transfect a broad range of cell types including suspension cells with superior efficiency. When tested against competitive transfection reagents OMNIfect transfection reagent benchmarks favorably against the market leaders. The table shown in the supporting data tab lists the cell lines in which OMNIfect has been shown to produce high transfection efficiency.Figure 2. Graph showing cell viability after transfection using OMNIfect transfection reagent in COS-1 cells compared with untreated cells as a control. Minimal cytotoxicityOMNIfect delivery reagent is very gentle on cells. Cytotoxicity was tested in COS-1 (Figure 2) cells and virtually no cell loss was observed. Cell viability following transfection was tested using CellTiter 96¢ç AQueous One Solution Cell Proliferation Assay according to the manufacturer¡¯s instructions. Data are presented as viability of OMNIfect treated cells relative to untreated cells.Get a free sample of OMNIfect with purchaseFor a limited time get a free sample of OMNIfect transfection reagent with any purchase of PLATINUM Select shRNA-mir or MGC premier cDNA clones. Add catalog number OTR1001 to your cart. At checkout please use promotion code SAMPLE to receive this 0.3ml aliquot of OMNIfect transfection reagent for free.Cell lines validated using OMNIfect Transfection Reagent |
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