In vivo siRNA

A new patented structure of siRNA with a special sequence motif provides Riboxx siRNAs with unique traits. The IVORI In Vivo siRNA is highly stable in serum and body fluids, more potent and less toxic than commonly used siRNAs. These properties make it ideal for gene silencing experiments in vivo (animal models) and preclinical studies.
 

 
 
Figure 1. IVORI siRNA with the proprietary riboxx sequence motif and typically introduced chemical modifications.
 
A new patented structure of siRNA with a special sequence motif (Figure 1) provides siRNA with unique traits. The IVORI siRNA is highly stable in serum and body fluids, more potent and less toxic than commonly used siRNAs. These properties make it ideal for gene silencing experiments in vivo (animal models) and preclinical studies.
Moreover, Riboxx provides scientists with the full range of siRNA delivery solutions, ranging from cholesterol groups to cell-penetrating peptides. Additional modifications in the backbone of the siRNA (e.g. in the sense strand) decrease the non-specififc immunologic response in vivo, subsequently increasing the specificity of gene silencing.
 

Effective Gene Silencing In Vivo

 
Figure 2. Inhibition of human Rhinovirus infection in mice by IVORI siRNA. IVORI siRNA targeting the genome of human Rhinovirus was applied at 1 mg/kg intra-nasally in BALB/6 mice. After 4 hours, a suspension of human Rhinovirus was inoculated intra-nasally in mice, and the viral load was measured after sacrifice of animals by qRT-PCR in the lower lobe of the left lung. As a control, an IVORI siRNA not targeting the viral genome was used. Measurements of the viral load were performed at the indicated time points and normalized to -actin.
 

Enhanced Serum Stability

 

Figure 3. Comparison of the stability of the IVORI siRNA and the siRNA with 3-overhangs in mouse serum. The IVORI siRNA was incubated at a concentration of 3 M in 80% mouse serum. Samples were analyzed at the indicated time points. Analysis was performed on 20% native PAGE and UV-transillumination. The IVORI siRNA displays stability in mouse serum for 72 h. While, the siRNA with 3-overhangs is digested at 48 h post-incubation.
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