![]() Features & Benefits• Á¤È®ÇÑ Á¤·® - hot-start±â´ÉÀ¸·Î primer-dimerÇü¼ºÀ» ÁÙÀÓ
• ¹Î°¨¼º - ÀûÀº ¾çÀÇ targetÀ¸·Î ÁõÆø °¡´É • ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë • ȣȯ¼º - fast cycling PCRÀ» Æ÷ÇÔÇÑ ¸ðµç real-time PCR±â°è¿Í ȣȯ • Protocol - fast qPCR °ú standard qPCR protocol ¸ðµÎ °¡´É Instrument CompatibilityABI 7500, 7500 FAST, ViiA7¢â, Stratagene (Agilent) Mx4000¢â, Mx3000P¢â, Mx3005P¢â (See product selection table), as well as other instruments that do not require the use of a passive reference.
DescriptionSensiFAST SYBR Kits´Â ºü¸£°í Àç»ý °¡´É °¡´ÉÇÑ real-time PCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. »ç¿ëÇϱâ ÆíÇϵµ·Ï SensiFAST SYBR Kit´Â 2X matermix typeÀ¸·Î Á¦°øµÇ¸ç real-time PCR¿¡ ÇÊ¿äÇÑ ¸ðµç SYBR Green I Dye, dNTPs, stabilisers¿Í enhancers±îÁö ¸ðµÎ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î reverse transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. 18~19pageÀÇ compatibility table ¿¡ ÀÖ´Â ±â°è¿¡¼ SYBR Green-based real-time PCR »ç¿ë¿¡ ÃÖÀûȵǾú½À´Ï´Ù. ÀÌ Å°Æ®´Â ¶ÇÇÑ ROX¸¦ »ç¿ëÇÏÁö ¾Ê´Â ´ÙÀ½ÀÇ ±â°è¿Íµµ ȣȯ °¡´ÉÇÕ´Ï´Ù. Qiagen(Corbett), Rotor-Gene 6000, Bio-Rad CFX 96, Roche LightCylcer 480) SensiFAST SYBR Hi-ROX¿Í Lo-ROX kitÀº ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. SensiFAST SYBR & Fluorescein Kit´Â ¼±Åà »ç¿ëÀ» À§ÇØ premixed FluoresceinÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. NotesSensiFAST is a trademark of Bioline Reagents Ltd. Components
Storage & StabilityAll kit components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR¢ç Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label. Shipping conditionsShipped on dry/blue ice. pi_50218_sensifast_sybr_lo_rox_kit_v1.pdf (434,086kb) |
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