"We were looking for a master mix to work on the LC480 platform. We use the SensiFAST probe No-ROX kit as it offers very good performance (in terms of sensitivity and fluorescence intensity) along with reduced costs."
- Institut für Mikrobiologie der Bundeswehr Neuherbergstr. 11 80937 München Features and Benefits• ¸ðµç non-specific À» ÃÖ¼ÒÈ ÇØÁÜÀ¸·Î½á Á» ´õ ³ªÀº È¿À²°ú º¸´Ù Å« Á¤È®µµ·Î ½ÇÇè °¡´É
• ´É·üÀûÀÎ multiplexing- throughputÀ» ³ôÀÌ°í ½ÇÇè ºñ¿ëÀ» ÁÙÀÌ¸é¼ sample ³²¿ëÀ» ÃÖ¼ÒÈ • ºü¸§ - fast cycling condition¿¡ ÃÖÀûȵǾî ÀÖ¾î high-throughput¿¡ ÀûÇÕÇÔ • °í°¨µµ - ÁõÆøÇϱ⠱î´Ù·Ó°Å³ª ÀûÀº ¾çÀÇ sample¿¡µµ »ç¿ëÇϱâ ÁÁÀ½ • ³ôÀº ½Å·Úµµ - ÃÖ°í ³ôÀº ÀçÇö¼º°ú ½Å·Úµµ¸¦ °¡Á®¿À±â À§ÇØ ¸¹Àº test¸¦ °Åħ • Protocol - fast qPCR °ú standard qPCR protocol ¸ðµÎ °¡´É Instrument CompatibilityRoche LightCycler¢ç 480 platform, Bio-Rad iCycler¢ç, iQ¢ç5, MyiQ¢â, Opticon¢â, Opticon¢â2, Chromo4¢â, MiniOpticon¢â, CFX96¢â, CFX384¢â, Cepheid SmartCycler¢ç, Qiagen Rotor-Gene¢â 3000, 6000, Eppendorf Mastercycler¢çep realplex, Illumina¢ç Eco¢â, Takara Thermal Cycler Dice¢ç (TP800) and Techne Quantica¢ç. See product selection table).
DescriptionSensiFAST Probe Kits´Â ºü¸£°í Àç»ý °¡´É °¡´ÉÇÑ real-time PCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. PrimeTime, TaqMan, Scorpions, Molecular Beacon probe¸¦ Æ÷ÇÔÇÑ probe-detection±â¼ú¿¡
»ç¿ëµÇµµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀºSensiFAST Probe kit°¡ ºü¸£°í Àç»ý°¡´ÉÇϸç ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ¶ÇÇÑ SensiFAST Probe´Âmultiplexing¿¡µµ ÀÌ»óÀûÀÔ´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î reverse transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. SensiFAST Probe Hi-ROX¿Í Lo-ROX kitÀº ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. VolumeBIO-86002: 250 x 20µl Reactions: 2 x 1ml
BIO-86005: 500 x 20µl Reactions: 5 x 1ml BIO-86020: 2000 x 20µl Reactions: 20 x 1ml Components2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs MgCl2Kit SizeThe kit size is based on a 20µl final reaction volume.
Storage Conditions12 months at -20¡ÆC. Avoid exposure of the ROX¢â to light.
NotesSensiFAST is a trademark of Bioline Reagents Ltd. protocol [pi_50204_sensifast_probe_no_rox_kit_v1.pdf] (434,106kb) |
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