CAT#	PRODUCT	SIZE	STORAGE	소비자가	주문
60602-2	E. cloni 5-alpha Chemically Competent Cells	24 rxns (DUOs)		-	로그인

E. cloni® 5-alpha Chemically Competent Cells

Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.).
Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.

E. cloni 5-alpha Chemically Competent Cells >1 × 10⁸ cfu/µg DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.

Table 1. E. cloni Competent Cells for Cloning

*E. cloni* Cell Lines	Transformation Efficiency (cfu/µg pUC DNA)	Cloning Methylated DNA	BAC, Cosmid Cloning	Blue/White Screening
5-alpha Chemically Competent	>1 × 10⁸ cfu/µg DNA	NO	NO	YES No IPTG induction required

Genotype

E. cloni 5-alpha: fhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

MA054-E-cloni-alpha-Chemically-Competent-Cells.pdf (194,774kb)

MSDS.pdf (118,131kb)