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DNA Oligonucleotide Resuspension and StorageIDT recommends resuspension in TE buffer rather than water. Oligonucleotides resuspended in water will have less stability and will have to be shipped on dry ice, which is more costly than standard shipping. Stability studies at IDT have shown that oligonucleotides stored in water at room temperature may show some degradation in as little as 60 days. The same studies showed that oligonucleotides stored dry or in TE at room temperature did not show signs of degradation for at least 7 months.
Starting Scale and YieldIDT uses a starting scale, referred to as just ¡°scale¡±, for the amount of initiating nucleotide used to begin oligo synthesis. However, processing steps such as cleavage and deprotection of the oligonucleotide will reduce the final delivery amount (yield). The addition of modifications, purification and length combinations also all affect the final yield of each oligo, which will always be less than the starting scale. The minimum yield guarantee is given in the shopping cart and in the order confirmation email. To receive more final yield, select a larger synthesis scale.
Purification and YieldAdditional purification significantly improves oligonucleotide performance in demanding applications such as multiplex PCR, cloning, mutagenesis, and antisense/RNAi methods. However, purification will always lower oligonucleotide final yield. HPLC routinely provides 85% purity, while PAGE provides 90% purity. The final yield of a PAGE purified oligo, however, will be less than an HPLC purified oligo. For example, a 250 nmole scale 25mer oligo will yield 8X with standard desalt, but only 2X with HPLC purification and 1X with Page purification.
Tools for Oligonucleotide Analysis and DesignIDT provides a number of free oligonucleotide design and analysis tools in the online SciTools¢ç suite. The suite is comprised of tools for designing assay components as well as determining the properties of any sequence entered. Check out the SciTools Suite website at www.idtdna.com/scitools
IDT Guarantees QualityIDT has pioneered the use of high throughput Quality Control methods and is the only oligonucleotide manufacturer that offers 100% QC and purity guarantees. Today, every oligo synthesized at IDT undergoes QC by mass spectrometry. IDT also offers CE and analytical HPLC as routine tools for additional QC on purified oligos. In addition to DNA, IDT has led the effort over the last decade to reduce the cost of high-quality custom RNA synthesis. Through improvements to the traditional tert-butyldimethylsilyl (tBDMS) chemistry and advances in instrumentation, IDT has achieved the highest coupling efficiency in the industry. Only at IDT are customers guaranteed the very best in quality synthesis with free mass spectrometry and CE traces on all purified oligos. |
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