MyTaq¢â DNA Polymerase
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-21105MyTaq DNA Polymerase500 UNITSat -20¡ÆC-·Î±×ÀÎ
BIO-21106MyTaq DNA Polymerase2500 UNITSat -20¡ÆC-·Î±×ÀÎ
BIO-21107MyTaq DNA Polymerase5000 UNITSat -20¡ÆC-·Î±×ÀÎ
-·Î±×ÀÎ
"Studying pollen-mediated gene flow means I have very limited sample volumes, MyTaq is the only polymerase I have found that can cope with this efficiently."

- Stirling University, Scotland

Features


• ¿ì¼öÇÑ performance¸¦ ÀÚ¶ûÇÏ´Â »õ·Î¿î polymerase
• dNTPs¿Í MgCl2¸¦ Æ÷ÇÔÇÑ µ¶Ã¢ÀûÀÎ buffer system
• ±¤¹üÀ§ÀÇ template¿¡ »ç¿ë °¡´ÉÇÏ¸ç ³ôÀº yield¿Í ¼º´ÉÀ» ÀÚ¶û
• ¹ÝÀÀ Áï½Ã gel loadingÀÌ °¡´ÉÇÏ°Ô Red dye Æ÷ÇÔ °¡´É
• Á» ´õ Æí¸®ÇÏ°Ô all-in-one master mix ŸÀÔÀ¸·Îµµ »ç¿ë °¡´É
 

Applications

 
• High-throughput PCR
• ºü¸¥ PCR ¹ÝÀÀ
• GC-rich sequence¿¡µµ °ß°íÇÑ ÁõÆø °¡´É
• Site-directed mutagenesis
• TA cloning °¡´É
 

Description


MyTaq¢â´Â Bioline¿¡¼­ »õ·Ó°Ô ¸¸µç ¾ß½ÉÀÛÀ¸·Î Æø³ÐÀº template- genomic DNA template¿¡µµ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù. DNA¿ÍÀÇ Ä£È­·ÂÀ» ³ô¿© ¼öÀ², °¨µµ, ¼Óµµ ¼¼°¡Áö Ãø¸éÀ» ¸ðµÎ Çâ»ó½Ãų ¼ö ÀÖ°Ô Á¦ÀÛµÈ Á¦Ç°ÀÔ´Ï´Ù. ƯÈ÷ enzyme°ú ÇÔ²² »õ·Ó°Ô upgrade µÈ Bioline¸¸ÀÇ µ¶Æ¯ÇÑ buffer·Î ÀÎÇØ º¹ÀâÇÑ template¿¡µµ ¹®Á¦¾ø´Â ±ò²ûÇÑ ÁõÆø °á°ú¸¦ Á¦°ø ÇÕ´Ï´Ù. UpgradeµÈ µ¶Æ¯ÇÑ bufferÀÇ Á¶¼ºÀº dNTPs¸¦ ºñ·ÔÇÏ¿© MgCl2,
enhancer°¡ ÀûÀýÇÏ°Ô ÃÖ»óÀÇ ³óµµ·Î ¼¯¿© optimization ¾øÀÌ Á¤È®ÇÑ ÁõÆøÀÌ °¡´ÉÇÏ°Ô ÇÕ´Ï´Ù.
MyTaq¢â Mix´Â ready-to-use 2X mix typeÀ¸·Î Á» ´õ ³ôÀº È¿À²¼º, throughput ±×¸®°í »ý»ê¼ºÀ» °®°Ô ÇØÁָ鼭 ºü¸¥ cycling condition·Î ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù. MyTaq¢â ¿ª½Ã red dye°¡ Æ÷ÇÔµÈ Á¦Ç°À¸·Î ±¸ÀÔÀÌ °¡´ÉÇÕ´Ï´Ù. PCR ÈÄ Ãß°¡·Î sample buffer¸¦ ³Ö¾î¼­ agarose gel¿¡
È®ÀÎÇÏ´Â ºÒÆíÇÔ ¾øÀÌ ¹Ù·Î loadingÀÌ °¡´ÉÇϱ⠶§¹®¿¡ ¸Å¿ì Æí¸®ÇÕ´Ï´Ù.
 

Components

MyTaq DNA Polymerase 5x MyTaq Reaction Buffer

Unit Definition


One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72¡ÆC.

 

Concentration


5u/¥ìl
 

Storage Conditions

 
MyTaq DNA Polymerase can be stored for up to 6 months at -20¡ÆC, or up to 2 weeks at +4¡ÆC. Repeated freeze/thaw cycles should be avoided.

 

Product Citations


Waldorf, K.M., et al. PLoS One 6(12), e28972 (2011). Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011). Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011).

 

Notes

 
MyTaq is a trademark of Bioline.
Fact sheet [MyTaq¢â_DNA_Polymerase.pdf] (549,393kb)
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