"Studying pollen-mediated gene flow means I have very limited sample volumes, MyTaq is the only polymerase I have found that can cope with this efficiently." - Stirling University, Scotland Features• ¿ì¼öÇÑ performance¸¦ ÀÚ¶ûÇÏ´Â »õ·Î¿î polymerase • dNTPs¿Í MgCl2¸¦ Æ÷ÇÔÇÑ µ¶Ã¢ÀûÀÎ buffer system • ±¤¹üÀ§ÀÇ template¿¡ »ç¿ë °¡´ÉÇÏ¸ç ³ôÀº yield¿Í ¼º´ÉÀ» ÀÚ¶û • ¹ÝÀÀ Áï½Ã gel loadingÀÌ °¡´ÉÇÏ°Ô Red dye Æ÷ÇÔ °¡´É • Á» ´õ Æí¸®ÇÏ°Ô all-in-one master mix ŸÀÔÀ¸·Îµµ »ç¿ë °¡´É Applications• High-throughput PCR • ºü¸¥ PCR ¹ÝÀÀ • GC-rich sequence¿¡µµ °ß°íÇÑ ÁõÆø °¡´É • Site-directed mutagenesis • TA cloning °¡´É DescriptionMyTaq¢â´Â Bioline¿¡¼ »õ·Ó°Ô ¸¸µç ¾ß½ÉÀÛÀ¸·Î Æø³ÐÀº template- genomic DNA template¿¡µµ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù. DNA¿ÍÀÇ Ä£È·ÂÀ» ³ô¿© ¼öÀ², °¨µµ, ¼Óµµ ¼¼°¡Áö Ãø¸éÀ» ¸ðµÎ Çâ»ó½Ãų ¼ö ÀÖ°Ô Á¦ÀÛµÈ Á¦Ç°ÀÔ´Ï´Ù. ƯÈ÷ enzyme°ú ÇÔ²² »õ·Ó°Ô upgrade µÈ Bioline¸¸ÀÇ µ¶Æ¯ÇÑ buffer·Î ÀÎÇØ º¹ÀâÇÑ template¿¡µµ ¹®Á¦¾ø´Â ±ò²ûÇÑ ÁõÆø °á°ú¸¦ Á¦°ø ÇÕ´Ï´Ù. UpgradeµÈ µ¶Æ¯ÇÑ bufferÀÇ Á¶¼ºÀº dNTPs¸¦ ºñ·ÔÇÏ¿© MgCl2, enhancer°¡ ÀûÀýÇÏ°Ô ÃÖ»óÀÇ ³óµµ·Î ¼¯¿© optimization ¾øÀÌ Á¤È®ÇÑ ÁõÆøÀÌ °¡´ÉÇÏ°Ô ÇÕ´Ï´Ù. MyTaq¢â Mix´Â ready-to-use 2X mix typeÀ¸·Î Á» ´õ ³ôÀº È¿À²¼º, throughput ±×¸®°í »ý»ê¼ºÀ» °®°Ô ÇØÁÖ¸é¼ ºü¸¥ cycling condition·Î ½ÇÇèÀÌ °¡´ÉÇÕ´Ï´Ù. MyTaq¢â ¿ª½Ã red dye°¡ Æ÷ÇÔµÈ Á¦Ç°À¸·Î ±¸ÀÔÀÌ °¡´ÉÇÕ´Ï´Ù. PCR ÈÄ Ãß°¡·Î sample buffer¸¦ ³Ö¾î¼ agarose gel¿¡ È®ÀÎÇÏ´Â ºÒÆíÇÔ ¾øÀÌ ¹Ù·Î loadingÀÌ °¡´ÉÇϱ⠶§¹®¿¡ ¸Å¿ì Æí¸®ÇÕ´Ï´Ù. ComponentsMyTaq DNA Polymerase 5x MyTaq Reaction BufferUnit DefinitionOne unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72¡ÆC. Concentration5u/¥ìl Storage ConditionsMyTaq DNA Polymerase can be stored for up to 6 months at -20¡ÆC, or up to 2 weeks at +4¡ÆC. Repeated freeze/thaw cycles should be avoided. Product CitationsWaldorf, K.M., et al. PLoS One 6(12), e28972 (2011). Cadar, D., et al. Virus Genes doi: 10.1007/s11262-011-0650-4 (2011). Cadar, D., et al. Arch. Virol. doi: 10.1007/s00705-011-1125-6 (2011). Notesprotocol [MyTaq¢â_DNA_Polymerase_protocol.pdf] (134,701kb) Fact sheet [MyTaq¢â_DNA_Polymerase.pdf] (549,393kb) |
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