transEDIT CRISPR-Cas9 Lentiviral reagents
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
CAHS1002transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAHS1001transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAHS1004transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAHS1003transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAMS1002transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAMS1001transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAMS1004transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CAMS1003transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CARS1002transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CARS1001transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CARS1004transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CARS1003transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCHS1002transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCHS1001transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCHS1004transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCHS1003transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCMS1002transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCMS1001transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCMS1004transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCMS1003transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCRS1002transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCRS1001transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCRS1004transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCRS1003transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent–80C-α
CCIHS1002transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIHS1001transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIHS1004transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIHS1003transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIMS1002transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIMS1001transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIMS1004transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIMS1003transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIRS1002transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIRS1001transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIRS1004transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CCIRS1003transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Bacterial Glycerol Stock3 constructs per target plus non-targeting control and transfection reagent. Inducible CAs9 plus transactivator–80C-α
CNHS2001transEDIT Human CRISPR Cas9 Nickase plus paired gRNA All-In-One target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control, tetracycline transactivator expression vector and transfection reagent–80C-α
CNMS2001transEDIT Mouse CRISPR Cas9 Nickase plus paired gRNA All-In-One target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control, tetracycline transactivator expression vector and transfection reagent–80C-α
CNRS2001transEDIT Rat CRISPR Cas9 Nickase plus paired gRNA All-In-One target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control, tetracycline transactivator expression vector and transfection reagent–80C-α
CNHS1001transEDIT Human CRISPR paired gRNA target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control and transfection reagent–80C-α
CNMS1001transEDIT Mouse CRISPR paired gRNA target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control and transfection reagent–80C-α
CNRS1001transEDIT Rat CRISPR paired gRNA target gene set (Custom) - Bacterial Glycerol Stock6 constructs per target plus non-targeting control and transfection reagent–80C-α
CAHV1002transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAHV1001transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAHV1004transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAHV1003transEDIT Human CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAMV1002transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAMV1001transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAMV1004transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CAMV1003transEDIT Mouse CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CARV1002transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CARV1001transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CARV1004transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CARV1003transEDIT Rat CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCHV1002transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCHV1001transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCHV1004transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCHV1003transEDIT Human CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCMV1002transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCMV1001transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCMV1004transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCMV1003transEDIT Mouse CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCRV1002transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Blast) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCRV1001transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-Puro) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCRV1004transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-tRFP) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CCRV1003transEDIT Rat CRISPR gRNA target gene set (pCLIP-gRNA-EFS-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control–80C-α
CIHV1002transEDIT Human Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Blast) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIHV1001transEDIT Human Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Puro) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIHV1004transEDIT Human Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-tRFP) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIHV1003transEDIT Human Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIMV1002transEDIT Mouse Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Blast) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIMV1001transEDIT Mouse Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Puro) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIMV1004transEDIT Mouse Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-tRFP) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIMV1003transEDIT Mouse Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIRV1002transEDIT Rat Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Blast) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIRV1001transEDIT Rat Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-Puro) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIRV1004transEDIT Rat Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-tRFP) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
CIRV1003transEDIT Rat Inducible CRISPR Cas9 Nuclease plus gRNA All-In-One target gene set (pCLIP-All-TRE3G-ZsGreen) - Viral Particles3 constructs per target plus non-targeting control, tetracycline transactivator expression vector–80C-α
TECC1001transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Puro)ea–80C-α
TECC1002transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-Blast)ea–80C-α
TECC1003transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-ZsGreen)ea–80C-α
TECC1004transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-EFS-tRFP)ea–80C-α
TECC1005transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Puro)ea–80C-α
TECC1006transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-Blast)ea–80C-α
TECC1007transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-ZsGreen)ea–80C-α
TECC1008transEDIT CRISPR Cas9 Nuclease Expression Vector (pCLIP-Cas9-Nuclease-hCMV-tRFP)ea–80C-α
TECN1001transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Puro)ea–80C-α
TECN1002transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-Blast)ea–80C-α
TECN1003transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-ZsGreen)ea–80C-α
TECN1004transEDIT CRISPR Cas9 Nickase Expression Vector (pCLIP-Cas9-Nickase-EFS-ZsGreen)ea–80C-α
TELA1001transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-Puro)ea–80C-α
TELA1002transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-Blast)ea–80C-α
TELA1003transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-ZsGreen)ea–80C-α
TELA1004transEDIT gRNA plus Cas9 Cloning Vector (pCLIP-All-EFS-tRFP)ea–80C-α
TELA1011transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Puro)ea–80C-α
TELA1012transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-Blast)ea–80C-α
TELA1013transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-ZsGreen)ea–80C-α
TELA1014transEDIT gRNA plus Cas9 Non-targeting Control (pCLIP-All-EFS-tRFP)ea–80C-α
TELG1002transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-Blast)ea–80C-α
TELG1003transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-ZsGreen)ea–80C-α
TELG1004transEDIT gRNA Cloning Vector (pCLIP-gRNA-EFS-tRFP)ea–80C-α
TELG1012transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-Blast)ea–80C-α
TELG1013transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-ZsGreen)ea–80C-α
TELG1014transEDIT gRNA Non-targeting Control (pCLIP-gRNA-EFS-tRFP)ea–80C-α

transEDIT CRISPR-Cas9 Lentiviral Reagents
Optimized gRNA designs, versatile expression vectors for efficient gene editing


transEDIT CRISPR-Cas9 reagents for gene editing include a range of lentiviral vectors expressing guide RNA (gRNA) and/or Cas9 nuclease or nickase.  The vectors are designed to select for high gene editing efficiency using direct indicators of CRISPR Cas9 expression from fluorescent or selection markers as well as efficient delivery as plasmid or lentiviral particles. In addition, complementary markers for co-selection of  Cas9 and gRNA ensure efficient expression of both components necessary for Cas9 based genome editing. 

  • Optimized gRNA designs cloned into your choice of gRNA or All-in-one (gRNA plus Cas9) lentiviral vectors
  • Single or paired guide RNA CRISPR strategies for gene editing 
  • Multiple vector configurations to enable dual or triple selection for enhanced efficiency
  • Constitutive Cas9 expression
  • Efficient delivery: transfect or transduce

Dont spend time cloning:
Search for your gene of interest using  FETCH my gene search, select your target gene set in choice of vector and format and add to your cart.  transOMIC technologies will select optimized designs for  the most 5' prime, high ranking gRNA with minimum off-targets for the most efficient gene knockout.  Bacterial glycerol stocks are shipped within two weeks.

CRISPR Cas9 Resources


CRISPR Cas9 Target gene sets are provided in a choice of vectors:
Target gene sets provide all of the gRNA vectors necessary for an experiment plus transfection reagent.  Select from the variety of vectors shown below and choose your format. 

  • 3 gRNA designs cloned in to your vector of choice
  • Negative gRNA Control
  • OMNIfect Transfection reagent


Lentiviral Expression Vector Options:

transEDIT CRISPR/Cas9 expression vectors have been constructed to provide flexibility with regards to the researchers experimental assay and the target cells of interest.   Guide RNA (gRNA) and Cas9 nuclease vectors are available as high-titer lentiviral vector particles or bacterial glycerol stocks. 

transEDIT CRISPR Cas9 Vectors have been designed with variable selectable markers so that multiple vectors can be delivered and selected for in the same cell. This has been shown to increase indel efficiency (Duda et al 2014). A number of promoter options are also offered to ensure optimal expression across a wide range of cell types.  

 
transEDIT Lentiviral Vectors - Transfect or Transduce for optimal editing
transEDIT All-in-One:

Cas9 and gRNA expressed from the same vector for added convenience.

gRNA plus Cas9 expression vectors
(pCLIP-All)
gRNA and Cas9 Lentiviral Expression Vectors for Genome Editing
Co-selection:

Optimize Cas9 and gRNA expression for your experiment with the compatible selection markers.

 
 
gRNA expression vectors
(pCLIP-gRNA)
gRNA Lentiviral Expression Vector for gene editing
Cas9 expression vectors
(pCLIP-Cas9 Nuclease, pCLIP-Cas9 Nickase)
CRISPR Cas9 Lentiviral Expression Vectors



Use to quickly find your gRNA Target Gene Set
Or

for paired gRNA and nickase options

Cas9 Vector Design - Selection for More Efficient Genome Editing


Figure 1. Fluorescent markers provide a method of enrichment for cells with increased indel frequency.    GFP expression was directly linked to Cas9 expression via 2A peptide.  FACS was used to bin cells into low medium and high expression of the fluorescent marker (and Cas9).  Indel frequency was measured using the CEL-I Surveyor assay and the percentages are shown at the bottom of each lane.  Cells enriched for the highest fluorescence expression showed the highest indel frequency.  
Cas9 Nuclease and Nickase Expression Vectors
The level of Cas9 endonuclease expression has been shown to affect the frequency of generating genome-edited clones. The efficiency of delivery and resulting expression of the targeting components vary widely between cell types.  Even within a given cell population, nuclease expression levels often vary significantly.  Consequently, only a small fraction of cells within culture express sufficient levels of Cas9 to facilitate double-strand break activity.

Duda et al 2014 showed that selection of high expressing Cas9 cells (using GFP or other markers as a fluorescent readout) resulted in a 4 to 20- fold increase in genome editing efficiency. Figure 1 shows the direct correlation between Cas9 expression level and indel frequency.
 
transEDIT Cas9 Nuclease expression vectors incorporate selection strategies to select for high Cas9 expressing cells and variable promoters to optimize expression in target cell lines. Tet-inducible Cas9 vectors have been developed to allow for more efficient control over Cas9 expression in your target cell line.
  • Transfect or transduce target cell lines with lentiviral Cas9 expression vectors- Transduction allows for efficient generation of Cas9 stable cell lines.
  • Multiple Selectable markers and Reporters - Blasticidin and/or puromycin to perform antibiotic selection selecting for Cas9 expressing cells. Co-selection can be performed with gRNA expression vectors for more efficient indel production. Fluorescent reporters  (ZsGreen or tRFP) in Cas9 vectors allow for FACS sorting of high expressing Cas9 cells.
  • Inducible Cas9 expression allows the creation of cell lines that limits constitutive expression of Cas9 which may reduce off target effects and may also be a necessary component when knockout of an essential gene is being considered.
  • Creation of stable cell lines with Cas9 or inducible Cas9 allows for more uniform expression of Cas9 which is desirable in the context of larger multiplexed screens.
  • Paired gRNA with Cas9 Nickase  for gene editing - Use Cas9 D10A mutant expressing vectors in combination with 2 gRNAs to produce efficient and specific gene editing (Ran et al., 2013).
transEDIT-Lentiviral-gRNA-_pCLIP-gRNA_-Target-Gene-Sets-_Bacterial-Glycerol-Stock_-Technical-Manual.pdf [transEDIT-Lentiviral-gRNA-_pCLIP-gRNA_-Target-Gene-Sets-_Bacterial-Glycerol-Stock_-Technical-Manual.] (706,190kb)
transEDIT-Lentiviral-gRNA-plus-Cas9-_pCLIP-All_-Target-Gene-Sets-_Viral-Particles_-Technical-Manual.pdf [transEDIT-Lentiviral-gRNA-plus-Cas9-_pCLIP-All_-Target-Gene-Sets-_Viral-Particles_-Technical-Manual.] (433,543kb)
transEDIT-Lentiviral-gRNA-plus-Cas9-_pCLIP-All_-Target-Gene-Sets-_Bacterial-Glycerol-Stock_-Technical-Manual.pdf [transEDIT-Lentiviral-gRNA-plus-Cas9-_pCLIP-All_-Target-Gene-Sets-_Bacterial-Glycerol-Stock_-Technica] (698,074kb)

transEDIT gRNA and Vector Design

Optimized gRNA designs produce efficient cleavage at the targeted site while limiting off-target effects.  transOMIC technologies uses the most current design algorithms to provide transEDIT gRNA designs with the higest ranked efficiency and lowest number of off-targets effects (Hsu et al. 2013, Wang et al. 2013). gRNA designs are provided as single gRNA for making double stranded breaks (DSBs) as well as paired gRNA for use with Cas9D10A (nickase) strategies.

transOMIC uses the following general guidelines for gRNA design and provides additional curation to select the top ranking gRNA:

(1) High efficiency of cleavage

  • Protospacer adjacent motif (PAM) - design sequences target 20 nucleotides of genomic sequence immediately upstream of the PAM sequence 5'-NGG
  • GC-content - between 45% and 80%
  • Nucleotide position effects - including the experimentally-determined effects of single and multiple mismatches on the targeting efficiency of off-target mismatches
  • Location within the gene -  target mutations closest to the start codon to ensure the best chance for loss of protein function

(2) Low off-target effects

  • Minimum possible homologous sites - select design with limited number of off-target sites in the genome
  • Evaluate off-target sites – penalize designs with off-target sites expected to be efficiently cleaved based on number and location of mismatches

Vector Specifications:

transEDIT gRNA expression vectors provide effective delivery to a wide range of cell types including non-dividing or post-mitotic cells.  Multiple selection options for use as a single-vector (All-in-one), in combination with transEDIT Cas9 nuclease vectors or in paired gRNA/nickase experiments. 

                transEDIT gRNA plus Cas9 Expression Vector

                         Convenience

  • Efficient delivery of gRNA and Cas9 in one vector
  • Single marker selection

                      transEDIT gRNA Expression Vector

               Modular for optimization

  • Control Cas9 expression in your target cell line (shown to be important for efficient knockout)
  • Complementary selection strategies for efficient co-expression with Cas9 or for paired gRNA/Cas9 Nickase strategy

To order transEDIT gRNA expression vectors:

1. Use  to quickly find your gRNA Target Gene Set in our standard gRNA or gRNA plus Cas9 (All-in-one) vectors.

2. For additional vector and format options select a form below:

                  1. Vector selection form for transEDIT single gRNA for use with Cas9 Nuclease
                  2. Vector selection form for transEDIT paired gRNA for use with Cas9 Nickase 

3. All vectors are available as cloning vectors

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PrimeTime qPCR 5' Nuclease Assays (Probe)
PrimeTime qPCR Primer Assays (SYBR)
Surveyor Mutation Detection Kits
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