SensiFAST SYBR Hi-ROX One-Step Kit
BIO-73001SensiFAST SYBR Hi-Rox One-Step100 RXNSat -20C-α
BIO-73005SensiFAST SYBR Hi-Rox One-Step500 RXNSat -20C-α
"After getting nowhere with other kits we tried the SensiFAST SYBR One-Step Kit and saw results first time, I cannot describe how wonderful this made us feel."

- Asia-Pacific Special Nutrients Sdn. Bhd.


Features and Benefits

• ΰ - Ȯ
• - ְ Ư̼ ΰ Ư
• Bio-Rad ȣȯ - dynamic well factor ϱ flourescein ʿ 迡
  (SensiFAST SYBR & Fluorescein kit)
• - one-tube ¾
• Protocol - fast qPCR standard qPCR protocol

Instrument Compatibility

ABI 7000, 7300, 7700, 7900, 7900HT, StepOne, StepOne Plus, each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.



SensiFAST SYBR One-step Kits RT-qPCR ߵǾ Ϲ ǰ ִ real-time 迡 Ǿϴ. SensiFAST SYBR One-Step Kits single tube first strand cDNA ļ real-time PCR ϵ Ǿϴ(fig 1). ֽ buffer enhancer ׸ antibody hot-start DNA Polymerase SensiFAST SYBR kit ְ ΰ real-time PCR(fig 2) ϵ մϴ. kit DNA template ϰ RNA template Bioline Tetro cDNA Synthesis Kit(BIO-65042) revere transcription մϴ. SensiFAST One-step kit 2X SensiFAST SYBR One-Step mix reverse transcriptase, RiboSafe Inhibitor Ǿ ֽϴ. SensiFAST SYBR Hi-Rox kit  û premixed ROX ԵǾ ֽϴ.


BIO-73001: 100 x 20µl Reactions: 1 x 1ml
BIO-73005: 500 x 20µl Reactions: 5 x 1ml



2x Reaction mix consisting of:
Heat-activated DNA polymerase
Ultra-pure dNTPs
SYBR Green I Plus separate vials of: Reverse transcriptase, RNase inhibitor, DEPC-treated water


Kit Size

The kit size is based on a 20µl final reaction volume.


Storage Conditions

12 months at -20C. Avoid exposure of the SYBR Green I to light.


Product Citations

Bergland A.O. et al. PLoS Genet. 8(4): e1002631(2012)



SensiFAST is a trademark of Bioline Reagents Ltd.

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SensiFAST SYBR Lo-ROX One-Step Kit
SensiFAST SYBR No-ROX One-Step Kit
SensiFAST SYBR & Fluorescein One-Step Kit