NEW! TransIT¢ç-Insect Transfection ReagentHigh yield transient transfection and baculovirus titers in insect cellsInsect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT¢ç-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types that offers:
Figure 1. Efficient Transfection of Baculovirus Genomic DNA using TransIT-Insect Reagent. Transfections were performed in 6-well plates with 5 x 105 Sf9 cells per well using TransIT-Insect Transfection Reagent at the reagent-to-total DNA ratio of 3:1 (µl:µg). Cells were co-transfected with 0.5 µg of ProGreen¢â baculovirus genomic vector DNA (AB Vector) encoding green-fluorescent protein (GFP) and 0.1 µg of pVL1393 transfer vector (AB Vector). (A) Fluorescence and phase contrast images were taken at 6 days post-transfection using a Zeiss S100 fluorescent microscope. Merge shown in (B).
Figure 2. TransIT-Insect Outperforms Competitor Transfection Reagents. Insect cell lines (A) Sf9, (B) High Five¢â, and (C) Drosophila S2 cells were transfected in 96-well plates with 0.1 µg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl: µg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.
Figure 3. TransIT-Insect Yields Increased Protein Expression Over Time. Insect cell lines (A) Sf9, (B) High Five¢â, and (C) Drosophila S2 were transfected in a 96-well plate with 0.1 ug of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the TransIT-Insect Transfection Reagent at a reagent-to-DNA ratio of 2:1 (µl: µg). Luciferase expression was measured at three time points, 24, 48 and 72 hours post-transfection. Sf9 and High Five cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.
Storage Conditions: Store at -20¡ÆC
Product Guarantee: 6 months |
»ùÇà ½ÅûÇϱâ
»ç¿ëÈıâ ÀÛ¼º
|