Features and Benefits
• ºü¸§ - ºü¸¥ reverse transcription real-time PCR¿¡ ÃÖÀûÈ
• Á¤È®ÇÑ Á¤·® - low copy RNA¿¡ ÃÖÀû • ¹Î°¨µµ - µ¶Æ¯ÇÑ buffer Á¶¼ºÀ¸·Î ºü¸¥ Ct °ª • ȣȯ¼º - ¸ðµç fast cycling instruments¿Í ȣȯ • Protocol - fast PCR °ú standard PCR protocol ¸ðµÎ °¡´É Instrument CompatibilityRoche LightCycler¢ç 480 platform, Bio-Rad Opticon¢â, Opticon¢â2, Chromo4¢â, MiniOpticon¢â, CFX96¢â, CFX384¢â, Cepheid SmartCycler¢ç, Qiagen Rotor-Gene¢â 3000, 6000, Eppendorf Mastercycler¢ç ep realplex and Techne Quantica¢ç. DescriptionSensiFAST Probe One-Step Kits´Â ºü¸¥ RT-qPCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. ÀÌ Å°Æ®´Â PrimeTime, TaqMan, Scorpions, Molecular Beacon probe¸¦ Æ÷ÇÔÇÑ probe-detection ±â¼ú»ç¿ë¿¡ ¶Ù¾î³ ¹Î°¨µµ¿Í ƯÀ̼ºÀ» °¡Áöµµ·Ï Á¶¼ºµÇ¾ú½À´Ï´Ù. SensiFAST SYBR One-Step Kits´Â single tube¿¡¼ ³ôÀº Àç»ý¼ºÀÇ first strand cDNA¿Í ÈÄ¼Ó real-time PCRÀÌ °¡´ÉÇϵµ·Ï Á¶¼ºµÇ¾ú°í ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST Probe kit°¡ ºü¸£°í Àç»ý°¡´ÉÇϸç ÃÖ°í·Î ¹Î°¨ÇÑ one step RT-qPCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. ¶ÇÇÑ SensiFAST Probe One-StepÀº multiplexing¿¡µµ ´ëÀûÇÒ ¼ö ¾ø´Â È¿À²¼ºÀ» º¸ÀÔ´Ï´Ù. SensiFAST Probe One-step kit´Â 2X SensiFAST Probe One-Step mix¿Í º°µµÀÇ reverse transcriptase, RiboSafe Inhibitor·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç SensiFAST Probe Hi-ROX¿Í Lo-ROX One-Step Kit´Â ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. VolumeBIO-76001: 100 x 20µl Reactions: 1 x 1ml BIO-76005: 500 x 20µl Reactions: 5 x 1ml Components2x Reaction mix consisting of:
Heat-activated DNA polymerase
Ultra-pure dNTPs
MgCl2
Plus separate vials of: Reverse Transcriptase, RNase inhibitor, DEPC-treated water
Kit SizeThe kit size is based on a 20µl final reaction volume. Storage Conditions12 months at -20¡ÆC. NotesSensiFAST is a trademark of Bioline Reagents Ltd. protocol [pi_50207_sensifast_probe_no_rox_one_step_kit_v1.pdf] (438,538kb) |
»ç¿ëÈıâ ÀÛ¼º
|