Features and Benefits• Ź¿ùÇÑ allele calling - ±ú²ýÇÑ discrimination • ÃÎÃÎÇÑ Çü±¤ cluster Çü¼º - template ¾çÀÌ ±ØÈ÷ Àû°Å³ª ¾î·Á¿î SNP loci¿¡ ¸Å¿ì ÀûÇÕ • ´õ »¡¶óÁø ½Ã°£ - ÇÏ·ç¿¡ ´õ ¸¹Àº ½ÇÇèÀ» °¡´ÉÇÏ°Ô ÇÔ • Dual-labeled probe ¿ëÀ¸·Î °³¹ß - IDT Primetime probe »ç¿ë Instrument CompatibilityThe SensiFAST¢â Genotyping Kit has been validated on commonly used real-time instruments. Hi-rox : ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne¢â, StepOne¢â Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
Lo-rox : ABI 7500, 7500 Fast, ViiA7¢â, QuantStudio¢â 12k Flex, Agilent Mx3000P¢â, Mx30005P¢â or Mx4000¢â (see product selection table) as well as other instruments that do not require the use of a passive reference.
DescriptionSensiFAST Genotyping Kit´Â ºü¸£°í Á¤È®ÇÏ°Ô ³ôÀº À翬¼ºÀ¸·Î sequence varients ¹× type IV SNPs±îÁö È®ÀÎ ÇÒ ¼ö ÀÖ½À´Ï´Ù. SensiFAST Genotyping Kit´Â ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀÌ SensiFAST Genotyping kit°¡ ºü¸£°í Àç»ý°¡´ÉÇϸç ÃÖ°í·Î ±ú²ýÇÑ allele clustering°ú discriminationÀ» Çϵµ·Ï ÇØ ÁÝ´Ï´Ù. PrimeTime, TaqMan, Scorpions, Molecular Beacon probe¸¦ Æ÷ÇÔÇÑ dual-labeled probe assay±â¼ú¿¡ »ç¿ëµÇµµ·Ï Á¶¼ºµÇ¾ú°í ÀϹÝÀûÀÎ real-time Àåºñ¿¡ ¸ðµÎ ȣȯ °¡´ÉÇÕ´Ï´Ù. SensiFAST Genotyping KitÀÇ Çâ»óµÈ buffer Á¶¼ºÀº allele specific probe°¡ ´õ¿í ƯÀÌÀûÀ¸·Î bindingÇÒ ¼ö ÀÖµµ·Ï ÇÏ¸ç °á°úÀûÀ¸·Î ´õ¿í ³¯Ä«·Î¿î melting temperature windows¸¦ È®ÀÎ ÇÒ ¼ö ÀÖ°í clearÇÑ allele cluster¸¦ ¸¸µé ¼ö ÀÖ½À´Ï´Ù. ÀÌ·¸°Ô ÃÖÀûÈµÈ protocol°ú ¹öÆÛ Á¶¼ºÀº ±Ã±ØÀûÀ¸·Î À翬¼º ³ô°í Á¤È®ÇÑ °á°ú¸¦ ´ë¿ë·® genotyping»ùÇÿ¡¼µµ º¸¿©ÁÙ ¼ö ÀÖ½À´Ï´Ù. VolumeKit Size
The kit size is based on a 20µl final reaction volume.
Storage Conditions12 months at -20¡ÆC. Avoid exposure of the ROX¢â to light. NotesSensiFAST is a trademark of Bioline. |
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