MBIOTECH.CO.KR :

CAT#	PRODUCT	SIZE	STORAGE	소비자가	주문
QT615-05	SensiMixPlus SYBR & Flu 500 rx	500 RXNS	at -20C°	-	로그인
QT615-20	SensiMix SYBR & Flourescein Kt	2000 RXNS	at -20C°	-	로그인
				-	로그인

"SensiMix products from Bioline are easy to use and give consistent results, they make the switch to real-time PCR very simple."

- Biobest Laboratories, The Edinburgh Technopole, Milton Bridge, Scotland.

Features and Benefits

Simple and reproducible: premixed with SYBR^® Green I and fluorescein, just add primers and template, reducing errors in set-up Specific and sensitive: for detection of a wide range of template concentration, even from hard to obtain and low copy number samples Flexible: suitable for block-based instruments where fluorescein is required to allow calculation of dynamic well factors 2x master mix: premixed with SYBR® Green I and an internal reference for convenience

Instrument Compatibility

Bio-Rad iCycler^®, iQTM5 and MyiQ™. (See product selection table). However the kit can is also compatible with several instruments that do not require the use of fluorescein, such as the Qiagen Rotor-Gene™ 6000, the Bio-Rad CFX96 or the Roche LightCycler^® 480.

Description

The SensiMix SYBR^® & Fluorescein Kit contains fluorescein, making it specifically suitable for use on real-time instruments that measure fluorescein signal, such as the Bio-Rad iCycler^®, iQ™5 and MyiQ™ platforms. Both SYBR^® Green I and fluorescein are premixed for immediate use.
With SYBR^® Green I and fluorescein in their premixed and optimized concentrations, as few as 10 copies of a target gene can be detected.

Volume

QT615-02: 250 x 50µl Reactions: 5 x 1.25ml
QT615-05: 500 x 50µl Reactions: 10 x 1.25ml
QT615-20: 2000 x 50µl Reactions: 40 x 1.25ml

Components

2x Reaction mix consisting of:

Heat-activated DNA polymerase

Ultra-pure dNTPs

MgCl₂ (6mM)

Fluorescein SYBR^® Green I

Plus separate vials of:
50mM MgCl₂

Kit Size

The kit size is based on a 50µl final reaction volume.

Storage Conditions

6 months at -20°C. Avoid exposure of the SYBR^® Green I to light.

Product Citations

Dhawan R. et al. Plant Cell 21: 1000-1019 (2009). Maatouk D.M. et al. Hum. Mol. Genet. 17(19): 2949-2955 (2008). Del Rosario B.C. & Pemberton L.F. Mol. Cell. Biol. 28(7): 2113-2124 (2008). Miller M.R. et al. J. Nutr. 138(11):2179-2185 (2008).

Notes

SensiMix is a trademark of Bioline Reagents Ltd.

protocol [pi_50230_sensimix_sybr_fluorescein_kit_v1.pdf] (433,480kb)