IDT
IDT
가장 많이 사용되는 Mid Range Prestained Protein Maker 입니다. 50 kDa 사이즈의 protein이 핑크색으로 염색되어 있고 나머지 protein들은 파란색으로 염색되어 있는 dual color marker 입니다. Sharp하고 정확한 recombinant protein으로 더욱 정확한 단백질의 사이즈를 측정할 수 있습니다.
DescriptionLandMarkTMmid range prestained protein marker is a dual color prestained size marker. It
is a mixture of 8 recombinant purified and prestained polypeptides whose
molecular weights are well-adjusted. It is available in a ready-to-use formula
(no need to boil before use), and is suitable for sizing proteins between
15,000 and 140,000 Da by SDS-PAGE. A blue and pink chromophore is covalently
bound to proteins, and 8 prestained proteins are visible during electrophoresis
or electrophoretic transfer from the gel to a membrane. The 50 KDa proteins
labeled with pink chromophore enable easy identification.
Because coupling of
chromophore to the proteins affects their apparent molecular weights in
SDS-PAGE, unstained protein standard should be used for accuracy >95%.
Features • Dual color size standard (blue and pink)
• Mid range size estimation
• Highly purified and blended 8 recombinant proteins
• His-tag sequence
in the proteins enables direct detection on western blots using anti-his
antibody
• User oriented ready to use solution
Recommended storage condition• -20℃ for one year
• Please
aliquot in a small volume for
prevention of repeated freezing & thawing
Usage information• Recommended loading volume:
5µl/lane for mini gel(10x8cm, 0.75 or 1mm thick)
10-15µl/lane for large gel(30x20cm, 1 or 1.5mm thick)
•Typical number of lanes: 100
•Number of Bands: 8
• Range (KDa): 15-140
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12% SDS-PAGE Gel, 5ul loading data