SensiFAST¢â HRM Kit
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-32005500 Reactions 2xSensiFAST HRM500 RXNSat -20¡ÆC-·Î±×ÀÎ
BIO-320202000 Reactions 2xSensiFAST HRM2000 RXNSat -20¡ÆC-·Î±×ÀÎ
-·Î±×ÀÎ
sensifast_6.jpg SensiFAST HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis.




 

Features & Benefits

• ºñ¿ë È¿À²Àû - Å« ½ºÄÉÀÏÀÇ genotyping ÇÁ·ÎÁ§Æ®¿¡ ÀÌ»óÀû
• °£´ÜÇϸ鼭 Àç»ý °¡´ÉÇÔ - ºñÀü°øÀÚ¿¡ ÀÇÇؼ­µµ °­·ÂÇÑ genotypingÀÌ °¡´É
• ¹Î°¨µµ - class 4(A/T) SNP mutationµµ °ËÃâ°¡´É
• ÃÖÀûÈ­µÈ ÇÁ·ÎÅäÄÝ - ÁõÆøÇϱ⠾î·Á¿î loci¿¡¼­µµ ½Å·Ú¼º ÀÖ´Â assay°¡ ºü¸£°Ô ¸¸µé¾îÁú ¼ö ÀÖÀ½
 

Instrument Compatibility

ABI 7300, 7500 FAST, Qiagen Rotor-Gene¢â 6000, Eppendorf Mastercycler¢ç ep realplex, Illumina¢ç Eco¢â, Roche LightCycler¢ç; 480, Bio-Rad Opticon¢ç, CFX96¢â, CFX384¢â, Idaho LightScanner¢ç 32 (LS32¢â), Takara Thermal Cycler Dice (TP800) (See product selection table).
 

Description

High Resolution Melt Curve(HRM) ºÐ¼®Àº nucleic acid»ùÇÃÀÇ Æ¯¼ºÀ» ±×µéÀÇ ºÐ¸® ¹æ½Ä¿¡ µû¶ó ºÐ¼®ÇÏ´Â °ÍÀ¸·Î intercalating dye, melt curve ºÐ¼®°ú Åë°èºÐ¼®ÀÌ Á¶ÇÕÀ» ÀÌ·ì´Ï´Ù. HRMÀº µÎ °¡´ÚÀÇ DNA°¡ ¿­·Î ºÐ¸®(melting)µÇ´Â ±âº»ÀûÀΠƯ¼º°ú fluorescent dye·Î ÀÌ meltingÀ» ¸ð´ÏÅ͸µ ÇÏ´Â °ÍÀ» ÀÌ¿ëÇÕ´Ï´Ù. SensiFAST HRM Kit´Â ºü¸£°í Àç»ý °¡´ÉÇÑ High Resolution Melt(HRM)ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï °³¹ß µÇ¾úÀ¸¸ç
ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â real-time±â°è¿¡¼­ °ËÁõµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST HRM kit°¡ ºü¸£°í Àç»ý °¡´ÉÇϸç ÃÖ°í·Î ¹Î°¨ÇÑ HRM ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. HRM ÀÇ ÁÖ¿ä Àû¿ëÀº Single Nucleotide Polymorphism(SNPs) genotyping(Fig 1), epigenetics(DNA methylation analysis), Zygosity testing(DNA mapping and DNA fingerprinting), gene scanning( PCR amplicons¿¡¼­ ¾Ë·ÁÁöÁö ¾ÊÀº variation À» ã´Â °Í) µî ÀÔ´Ï´Ù.

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.

Components

Reagent
200 x 20¥ìl reactions
500 x 20¥ìl reactions
2000 x 20¥ìl reactions
SensiFAST HRM  mix (2x)
2 x 1ml
5 x 1ml
20 x 1ml

Volume

BIO-32002: 200 x 20¥ìl Reactions: 2 x 1ml
BIO-32005: 500 x 20¥ìl Reactions: 5 x 1ml
BIO-32020: 2000 x 20¥ìl Reactions: 20 x 1ml

Storage & Stability

All kit components should be stored at -20¡ÆC upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Avoid exposure of the EvaGreen to light.

Shipping conditions

SensiFAST HRM Kit is shipped on dry/blue ice.
protocol [pi_50192_sensifast_hrm_kit_v1.pdf] (385,683kb)
Fact sheet [sensifast_hrm_kit_factsheet.pdf] (690,657kb)
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