SensiFAST HRM uses the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure that the SensiFAST HRM Kit delivers fast, reproducible, highly-specific and ultra-sensitive HRM analysis.
Features & Benefits• ºñ¿ë È¿À²Àû - Å« ½ºÄÉÀÏÀÇ genotyping ÇÁ·ÎÁ§Æ®¿¡ ÀÌ»óÀû• °£´ÜÇÏ¸é¼ Àç»ý °¡´ÉÇÔ - ºñÀü°øÀÚ¿¡ ÀÇÇؼµµ °·ÂÇÑ genotypingÀÌ °¡´É • ¹Î°¨µµ - class 4(A/T) SNP mutationµµ °ËÃâ°¡´É • ÃÖÀûÈµÈ ÇÁ·ÎÅäÄÝ - ÁõÆøÇϱ⠾î·Á¿î loci¿¡¼µµ ½Å·Ú¼º ÀÖ´Â assay°¡ ºü¸£°Ô ¸¸µé¾îÁú ¼ö ÀÖÀ½ Instrument CompatibilityABI 7300, 7500 FAST, Qiagen Rotor-Gene¢â 6000, Eppendorf Mastercycler¢ç ep realplex, Illumina¢ç Eco¢â, Roche LightCycler¢ç; 480, Bio-Rad Opticon¢ç, CFX96¢â, CFX384¢â, Idaho LightScanner¢ç 32 (LS32¢â), Takara Thermal Cycler Dice (TP800) (See product selection table).
DescriptionHigh Resolution Melt Curve(HRM) ºÐ¼®Àº nucleic acid»ùÇÃÀÇ Æ¯¼ºÀ» ±×µéÀÇ ºÐ¸® ¹æ½Ä¿¡ µû¶ó ºÐ¼®ÇÏ´Â °ÍÀ¸·Î intercalating dye, melt curve ºÐ¼®°ú Åë°èºÐ¼®ÀÌ Á¶ÇÕÀ» ÀÌ·ì´Ï´Ù. HRMÀº µÎ °¡´ÚÀÇ DNA°¡ ¿·Î ºÐ¸®(melting)µÇ´Â ±âº»ÀûÀΠƯ¼º°ú fluorescent dye·Î ÀÌ meltingÀ» ¸ð´ÏÅ͸µ ÇÏ´Â °ÍÀ» ÀÌ¿ëÇÕ´Ï´Ù. SensiFAST HRM Kit´Â ºü¸£°í Àç»ý °¡´ÉÇÑ High Resolution Melt(HRM)ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï °³¹ß µÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â real-time±â°è¿¡¼ °ËÁõµÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST HRM kit°¡ ºü¸£°í Àç»ý °¡´ÉÇϸç ÃÖ°í·Î ¹Î°¨ÇÑ HRM ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. HRM ÀÇ ÁÖ¿ä Àû¿ëÀº Single Nucleotide Polymorphism(SNPs) genotyping(Fig 1), epigenetics(DNA methylation analysis), Zygosity testing(DNA mapping and DNA fingerprinting), gene scanning( PCR amplicons¿¡¼ ¾Ë·ÁÁöÁö ¾ÊÀº variation À» ã´Â °Í) µî ÀÔ´Ï´Ù. NotesSensiFAST is a trademark of Bioline Reagents Ltd. Components
VolumeBIO-32002: 200 x 20¥ìl Reactions: 2 x 1mlBIO-32005: 500 x 20¥ìl Reactions: 5 x 1ml BIO-32020: 2000 x 20¥ìl Reactions: 20 x 1ml Storage & StabilityAll kit components should be stored at -20¡ÆC upon receipt. When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date on the outer box label. Avoid exposure of the EvaGreen to light. Shipping conditionsSensiFAST HRM Kit is shipped on dry/blue ice. protocol [pi_50192_sensifast_hrm_kit_v1.pdf] (385,683kb) Fact sheet [sensifast_hrm_kit_factsheet.pdf] (690,657kb) |
»ç¿ëÈıâ ÀÛ¼º
|