ReadyMade™ Buffers and Solutions
CAT# PRODUCT SIZE STORAGE 소비자가 주문
11-01-02-0210 x 2 ml IDTE pH 7.5 (1X TE Solution)10 x 2 ml4C-로그인
11-01-02-0510 x 2 ml IDTE pH 8.0 (1X TE Solution)10 x 2 ml4C-로그인
11-05-01-15300 mL IDTE pH 7.5 (1X TE Solution)300 mL4C-로그인
11-05-01-13300 mL IDTE pH 8.0 (1X TE Solution)300 mL4C-로그인
11-05-01-051 Liter IDTE pH 7.5 (1X TE Solution)1 Liter4C-로그인
11-05-01-091 Liter IDTE pH 8.0 (1X TE Solution)1 Liter4C-로그인
11-05-02-034 x 1 Liter IDTE pH 7.5 (1X TE Solution)4 x 1 Liter4C-로그인
11-05-02-044 x 1 Liter IDTE pH 8.0 (1X TE Solution)4 x 1 Liter4C-로그인
11-05-01-01250 mL Nuclease Decontamination Solution250 mL4C-로그인
11-05-03-016 x 250 mL Nuclease Decontamination Solution6 x 250 mL4C-로그인
11-04-02-0110 x 2ml Nuclease Free Water10 x 2 ml4C-로그인
11-05-01-14300 mL Nuclease Free Water300 mL4C-로그인
11-05-01-041 Liter Nuclease Free Water1 Liter4C-로그인
11-05-02-024 x 1 Liter Nuclease Free Water4 x 1 Liter4C-로그인
11-01-03-0110 x 2 ml Nuclease Free Duplex Buffer10 x 2 ml4C-로그인
11-05-01-12300 mL Nuclease Free Duplex Buffer300 mL4C-로그인
11-05-01-031 Liter Nuclease Free Duplex Buffer1 Liter4C-로그인
11-05-02-014 x 1 Liter Nuclease Free Duplex Buffer4 x 1 Liter4C-로그인

Buffers and Solutions

 
While many researchers continue to store oligonucleotides in water, resuspension in a buffered solution such as TE is recommended. DNA oligonucleotides can be damaged by prolonged incubation or storage in even mildly acidic solutions; DNA dissolved in distilled water will often have a final pH <5.0 and is at risk for depurination. RNA will degrade in alkaline conditions. Further, single-stranded nucleic acids can easily be degraded by trace contamination with a variety of nucleases that are common contaminants in a normal laboratory environment. Any solution used to resuspend and store DNA or RNA should be nuclease-free. It is recommended that stock oligo solutions be made at high concentration and stored frozen. More dilute working solutions can be made from the stocks at intervals as needed. The following solutions are for resuspension and dilution of oligos. Solutions are guaranteed to be nuclease-free. Each lot is tested using our RNaseAlert® and DNaseAlert™ reagents to document the absence of any detectable nuclease activity. Individual lots are screened for endotoxins using a Limulis Abocyte Lysate (LAL) assay.
 
 

IDTE (10 mM Tris, pH 7.5 or 8.0, 0.1 mM EDTA)

A 1x TE buffer for initial resuspension and storage of DNA oligos
 
Description
Part # Pricing
10 x 2 ml IDTE pH 7.5 (1X TE Solution) 11-01-02-02 ₩36,000
10 x 2 ml IDTE pH 8.0 (1X TE Solution) 11-01-02-05 ₩36,000
300 mL IDTE pH 7.5 (1X TE Solution) 11-05-01-15 ₩36,000
300 mL IDTE pH 8.0 (1X TE Solution) 11-05-01-13 ₩36,000
1 Liter IDTE pH 7.5 (1X TE Solution) 11-05-01-05 ₩60,000
1 Liter IDTE pH 8.0 (1X TE Solution) 11-05-01-09 ₩60,000
4 x 1 Liter IDTE pH 7.5 (1X TE Solution) 11-05-02-03 ₩190,000
4 x 1 Liter IDTE pH 8.0 (1X TE Solution) 11-05-02-04 ₩190,000

Nuclease Decontamination Solution

 
Many RNases and DNases are naturally found in the environment. Further, a variety of nucleases are employed in routine molecular biology methods and can accidentally contaminate lab surfaces at very high levels. In particular, RNases can be very difficult to eliminate and can cause damage to projects that require manipulations of intact RNA samples, such as functional genomics experiments, real-time quantitative PCR, microarrays, etc.
Nuclease Decontamination Solution irreversibly inactivates nucleases and can be applied to most lab surfaces to remove nuclease contamination. Just spray, rinse and let dry. Nuclease Decontamination Solution eliminates the need to bake glassware and can be applied to plastic surfaces which are difficult to sterilize.
 
Description
Part # Pricing
250 mL Nuclease Decontamination Solution 11-05-01-01 ₩70,000
6 x 250 mL Nuclease Decontamination Solution 11-05-03-01 ₩330,000

Nuclease-free Water

For initial resuspension and storage of single-stranded RNA oligos. Also suitable for making dilute working solutions from stock oligos
 
Description
Part # Pricing
10 x 2ml Nuclease Free Water 11-04-02-01 ₩32,000
300 mL Nuclease Free Water 11-05-01-14 ₩32,000
1 Liter Nuclease Free Water 11-05-01-04 ₩56,000
4 x 1 Liter Nuclease Free Water 11-05-02-02 ₩180,000

Duplex Buffer (30 mM HEPES, pH 7.5, 100 mM Potassium Acetate)

For initial resuspension, annealing, and storage of duplex RNAi products.
 
Description
Part # Pricing
10 x 2 ml Nuclease Free Duplex Buffer 11-01-03-01 ₩36,000
300 mL Nuclease Free Duplex Buffer 11-05-01-12 ₩36,000
1 Liter Nuclease Free Duplex Buffer 11-05-01-03 ₩60,000
4 x 1 Liter Nuclease Free Duplex Buffer 11-05-02-01 ₩190,000
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