Nuclease Detection Products
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
alertRNaseAlert Substrate - (25) single-use tubes [50 pmole]25 single use- 20C-α
RNaseAlert SubstRNaseAlert Substate (bulk tube) [2nmole]2 nmole- 20C-α
RNaseAlert[IDT]RNaseAlert KitKit- 20C-α
10x RNaseAlert10x RNaseAlert Buffer [2ml]2 ml- 20C-α
10x DNaseAlert10x DNaseAlert Buffer [2ml]2 ml- 20C-α
DNaseAlert[IDT]DNaseAlert KitKit- 20C-α
DNaseAlert SubstDNaseAlert Substate (bulk tube) [2nmol]2 nmole- 20C-α
DalertDNaseAlert Substrate - (25) single-use tubes [50 pmole]25 single use- 20C-α
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Nuclease Detection Products

 
IDT RNases DNases detection Kit ΰϰ ֽϴ. Nuclease degradation Ÿ ϴ Ȯ ֽϴ. assay enzyme kinetics ֽϴ.
 
 
Nucleases are widely present in the laboratory environment and can interfere with many experiments. In particular, single-stranded RNases are ubiquitous, hard to eliminate, and can rapidly degrade important samples used in microarray studies, real-time PCR, northern blots, or cDNA cloning.
 
IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescence signal only after nuclease degradation. The assay can be read visually or measured and quantified using fluorometry. Assays can be used qualitatively to test lab reagents, equipment, and supplies for nuclease contamination. Assays can be used quantitatively to study enzyme kinetics. Two oligonucleotide substrates are available: one designed to detect RNases and the second designed to detect DNases. The RNaseAlert substrate employs a FAM reporter (Em 520 nm). The DNaseAlert substrate employs a HEX reporter (Em 555 nm).
 


RNaseAlert and DNaseAlert are available as:
Single-use tubes (25 tubes with 0.05 nmole each)—ideal for rapid hand-held visual assays at the research bench
Bulk substrate (2 tubes with 2 nmole each)—for use in either visual assays or read in a fluorometer
Kit—includes single use tubes, buffer, water, enzyme, and nuclease decontamination solution

RNaseAlert Kit Components:

 
25 50 pmole Fluorescent Substrate tubes
250 µL RNaseAlert Buffer
2 mL Nuclease-Free water
50 µL RNase A
50 mL Nuclease Decontamination Solution
Kit quality guaranteed for six months from date received when stored at –20C.
 

DNaseAlertKit Components:

 
25 50 pmole Fluorescent Substrate tubes
250 µL DNaseAlert Buffer
2 mL Nuclease-Free water
25 µL 2U/mL DNase 1 Enzyme
50 mL Nuclease Decontamination Solution
 
Kit quality guaranteed for six months from date received when stored at –20C.
 
RNase Alert.jpg

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rU RNase Substrate
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