MiraCLEAN¢ç Endotoxin Removal Kit
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
MIR5910[Mirus]MiraCLEAN¢ç Endotoxin Removal Kitfor 10 mg DNA-·Î±×ÀÎ
MIR5900[Mirus]MiraCLEAN¢ç Endotoxin Removal Kitfor 100 mg DNA-·Î±×ÀÎ
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MiraCLEAN¢ç Endotoxin Removal Kit

A convenient and effective method for the removal of endotoxin from plasmid DNA preparations

  • Effectively Removes Endotoxin from Plasmid Samples - Aids in the safety and efficiency of gene delivery research.
  • Compatible With In Vivo and In Vitro Applications - Removes harmful endotoxin that could cause decreased transfection efficiencies in vitro and inflammatory reactions in vivo .
  • Ease of Use - Simple phase separation protocol allows quick visualization of recovered nucleic acid preparations.
Figure 1. The MiraCLEAN¢ç Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per mg of DNA (EU/mg). The DNA was treated with the MiraCLEAN¢ç Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
 
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Figure 2. Plasmid DNA Exhibits High Transfection Efficiency after Treatment with the MiraCLEAN Kit. COS-7 cells were transfected with 2 µg of plasmid DNA and 2µl of the TransIT¢ç-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression.
Storage Conditions: Store EndoGO Extraction Reagent at 4¨¬C (warm to room temperature and vortex before use), store MiraCLEAN¢ç
Product Guarantee: 1 year

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