Biotinylation

Biotinylation

Biotin-modified oligos bind tightly to streptavidin. The streptavidin can be labeled with fluorescent dyes and enzymes or mediate attachment to a solid surface. A variety of molecular biology assays and purification methods employ biotin. Biotin can be added to the 5- or 3-ends of an oligo using either a C6 (standard) or TEG (tetra-ethyleneglycol, 15 atom) spacer arm. 5' Biotin-TEG requires purification. Internal biotin modification can be introduced using a biotin dT base, which also requires additional purification.
 
Scientific Details
MW: 393.4
Mod Code: /5Biosg/
 
 
 
 
 
Scientific Details
MW: 437.4
Mod Code: /3Bio/
 
 
 
 
 
Structure
Structure
 
 
 

 
Biotin (Azide) is attached to the oligo sequence through a 1,2,3-triazole linkage. The 1,2,3-triazole linkage is formed from the reaction of azides and alkynes in the presence of copper (I), in the click reaction. The internal version of this modification is attached to the oligo through a dT base. Incorporation of the internal version will add a dT nucleotide at that position. To avoid adding an extra nucleotide, replace an existing T nucleotide in your sequence with the required modification.
 
Scientific Details
MW: 486.5
Mod Code: /5BioK/
 
 
 
 
 
 
Scientific Details
MW: 430.6
Mod Code: /iBiodUK/
 
 
 
 
 
Structure
Structure
 
Scientific Details
MW: 380.5
Mod Code: /5BiodT/
 
 
 
 
 
Scientific Details
MW: 380.5
Mod Code: /iBiodT/
 
 
 
 
 
Scientific Details
MW: 380.5
Mod Code: /3BiodT/
 
 
 
 

Structure
Structure
 
 

 
Biotin-TEG has an extended spacer arm.
 
Scientific Details
MW: 481.5
Mod Code: /5BiotinTEG/
 
 
 
 
 
Scientific Details
MW: 569.6
Mod Code: /3BioTEG/
 
 
 
 
Structure
Structure
 
 

 
Two biotin groups are sequentially placed on the 5-end, which increases the efficiency of streptavidin binding.
 
Scientific Details
MW: 871
Mod Code: /52-Bio/
 
 
 
 
Structure
Structure
 
 

 
PC Biotin products a photocleavable spacer between the biotin group and the DNA bases. Optimal cleavage is obtained with exposure to long-wave UV light in the 300-350 nm spectral range. Cleavage releases the oligo with a 5'-phosphate group.
 
Scientific Details
MW: 597.6
Mod Code: /5PCBio/
 
 
 
 
Structure
Structure
 
 

 
Desthiobiotin is a biotin analog that is missing the sulfur atom. Its affinity to streptavidin is lower than that of biotin. Displacement of desthiobiotin to streptavidin can occur by simply adding biotin to a reaction mixture.
 
Scientific Details
MW: 539.6
Mod Code: /5deSBioTEG/
 
 
 
 
 
Scientific Details
MW: 539.6
Mod Code: /ideSBioTEG/
 
 
 
 
 
Scientific Details
MW: 539.6
Mod Code: /3deSBioTEG/
 
 
 
 
Structure
Structure
 
 
 
 
 
 
 
 
 

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