Thiol Modifications
A thiol group can be used to attach an oligo to a variety of fluorescent and nonfluorescent moieties or surfaces. Oligos containing thiol modifiers are shipped in their oxidized (disulfide) form and require chemical reduction by dithiothreitol (DTT) or Tris (2-carboxyethyl) phosphine (TCEP) prior to use.
 
Scientific Details
MW: 244.3
Mod Code: /3ThioMC3-D/
 
 
 
 
 
Structure
 
Structure
 
 

 
Dithiol can be inserted into an oligonucleotide at the 5' position, the 3' position or internally. Each insertion results in 2 SH group available for coupling with ligands or surfaces (linkage to gold surfaces in one popular application). The DTPA modification can be inserted in series so that 2 or even 3 groups can be positioned adjacent to each other to increase efficiency of ligand/surface interactions. Note that serial insertions will decrease oligo yield due to lower efficiency of incorporation of the DTPA phosphoramidite.
 
Scientific Details
MW: 214.2
Mod Code: /5DTPA/
 
 
 
 
 
Scientific Details
MW: 214.2
Mod Code: /iDTPA/
 
 
 
 
 
Scientific Details
MW: 214.2
Mod Code: /3DTPA/
 
 
 
 
 
Structure
Structure
 
 
 

Scientific Details
MW: 328.4
Mod Code: /5ThioMC6-D/
 
 
 
 
 
Structure
 
Structure
 

 
 
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