A post-deprotection reaction is used to attach this fluorophore to the oligonucleotide. This is commonly used to modify RNA oligos with FAM.

 

Extinction Coefficient: 15700

- at Absorbance Max: 83000

 

A single isomer derivative of fluorescein. FAM is the most commonly used fluorescent dye attachment for oligonucleotides and is compatible with most fluorescence detection equipment. It becomes protonated and has decreased fluorescence below pH 7; it is typically used in the pH range 7.5–8.5. FAM can be attached to 5' or 3' end of oligos.

 

Extinction Coefficient: 20960

- at Absorbance Max: 75000

   

 

Fluorescein is attached to position 5 of the thymine ring by a 6-carbon spacer arm.

 

Extinction Coefficient: 13700

- at Absorbance Max: 75000

      

 

 

This modification is attached to the oligo through a dT base. Incorporation of this modification will add a dT nucleotide at the 3¡¯ end of your oligo.

 

Extinction Coefficient: 31980

- at Absorbance Max: 89000

   

 

 

Most often used to multiplex with FAM. It can be used as a VIC¢â substitute because its spectral profile is nearly identical to VIC¢â. Importantly, MAX is efficiently excited by either a 488 nm laser or a broad emission light source; therefore, it can be used with most commercial detection platforms.

 

Extinction Coefficient: N/A

- at Absorbance Max: 108000

Alternative:

JOE (NHS Ester)

-Prices starting at $273,000.00 KRW

Tetrachlorofluorescein is a chemical relative of fluorescein that is commonly used for multiplexed assays with FAM and HEX. TET can be added only to the 5' end of an oligonucleotide.

 

Extinction Coefficient: 16300

- at Absorbance Max: 86000

      

 

 

ROX (carboxy-X-rhodamine) is available only as an NHS ester; therefore, it must be attached to an amino-modified oligonucleotide. It has spectral properties similar to Texas Red¢ç.

 

Extinction Coefficient: 22600

- at Absorbance Max: 82000

       

 

 

 

A bright fluorescent dye that can be used in microarray applications. TYE 563 can be used as a direct substitute for Cy3¢ç.

 

Extinction Coefficient: 10000

- at Absorbance Max: 137800

 

 

Hexachlorofluorescein is a chemical relative of fluorescein that is commonly used for multiplexed assays with FAM and TET. HEX can be added only to the 5' end of an oligonucleotide.

 

Extinction Coefficient: 31600

- at Absorbance Max: 96000

     

 

 

A red wavelength dye commonly used in multiplex reactions with FAM or in fluorescence-activated cell sorting (FACS) and microscopy applications using the rhodamine filter. TEX 615 can be used as a direct substitute for Texas Red or CAL Fluor 610.

 

Extinction Coefficient: 10700

- at Absorbance Max: 88600

 

 

A bright fluorescent dye that can be used in microarray applicaitons. TYE 665 can be used as a direct substitute for Cy5¢ç.

 

Extinction Coefficient: 35700

- at Absorbance Max: 293800

 

A bright fluorescent dye that can be used in microarray applications. TYE 705 can be used as a direct substitute for Cy5.5¢ç.

 

Extinction Coefficient: 56800

- at Absorbance Max: 275300