6-FAM (Azide)

This form of FAM is attached to the oligonucleotide using click chemistry. The internal version of this modification is attached to the oligo through a dT base. A dT nucleotide will be added at the position of the modification. To avoid adding an extra nucleotide, replace an existing T nucleotide in your sequence with the required modification.

Extinction Coefficient: 15700

- at Absorbance Max: 83000

Texas Red®-X (NHS Ester)

Texas Red®-X is a commonly used red wavelength fluorescent dye. It is available only as an NHS ester and must be attached to an amino-modified oligonucleotide.

Extinction Coefficient: 14400

- at Absorbance Max: 80000

Lightcycler® 640 (NHS Ester)

Lightcycler® 640 (NHS Ester) is only available as an NHS Ester and must be attached to an amino-modified oligonucleotide. It is a red dye which spectrally lies between Texas Red® (NHS Ester) and Cy5™. It is commonly used as a FRET reporter along with a 6-FAM™ donor in assays run on the Roche Lightcycler® real-time PCR platform.

Extinction Coefficient: 67363

- at Absorbance Max: 181070

Dy 750 (NHS Ester)

Extinction Coefficient: 18900

- at Absorbance Max: 270000