Iowa Black® Dark Quenchers
The Iowa Black® (patent pending) quenchers are a new family of dark quenchers developed by IDT that are offered as either 5'- or 3'-end modifications. They have excellent properties for use in fluorescence quenched probes. These compounds are stable over a wide range of conditions, including pH and heat. Further, they provide a convenient hydrophobic handle which facilitates purification by HPLC methods.

Iowa Black® FQ has a broad absorbance spectra ranging from 420 to 620 nm with peak absorbance at 531 nm. This quencher is ideal for use with fluorescein and other fluorescent dyes that emit in the green to pink spectral range.
 
Scientific Details
MW: 442.4
Mod Code: /5IABkFQ/
Extinction Coefficient: 13344
Absorbance Max: 531 nm
Extinction Coefficient
(At Absorbance max):
38216
 
 
 
 
 
 
 
 
 
Scientific Details
MW: 442.4
Mod Code: /3IABkFQ/
Extinction Coefficient: 13344
Absorbance Max: 531 nm
Extinction Coefficient
(At Absorbance max):
38216
 
 
 
 
 
 
 
 
 
 

 
Iowa Black® RQ has a broad absorbance spectra ranging from 500 to 700 nm with peak absorbance at 656 nm. This quencher is ideal for use with Texas Red®, Cy5TM, and other fluorescent dyes that emit in the red spectral range.
 
Scientific Details
MW: 420.4
Mod Code: /5IAbRQ/
Extinction Coefficient: 50457
Absorbance Max: 656 nm
Extinction Coefficient
(At Absorbance max):
18782
 
 
 
 
 
 
 
 
 
Scientific Details
MW: 678.6
Mod Code: /3IAbRQSp/
Extinction Coefficient: 44506
Absorbance Max: 667 nm
Extinction Coefficient
(At Absorbance max):
20022
 
 
 
 
 
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