Dark Quenchers Modifications

BHQ-1 is best suited for fluorophores that emit in the wavelength range from FAMTM to TAMRATM.
 
Scientific Details
MW: 554.5
Mod Code: /3BHQ_1/
Extinction Coefficient: 8000
Absorbance Max: 534 nm
Extinction Coefficient
(At Absorbance max):
34000
 
 
 
 
 
 
 
 
 

 
BHQ-2 is best suited for fluorophores in the Cy3TM to Cy5TM wavelength range.
 
Scientific Details
MW: 556.5
Mod Code: /3BHQ_2/
Extinction Coefficient: 8000
Absorbance Max: 578 nm
Extinction Coefficient
(At Absorbance max):
38000
 
 
 
 
 
 
 
 
 

 
Dabcyl is a relatively weak dark quencher and is best suited for use in Molecular Beacons where the hairpin places the quencher and fluorescent dye in close proximity.
 
Scientific Details
MW: 468.6
Mod Code: /3Dab/
Extinction Coefficient: 7283
Absorbance Max: 478 nm
Extinction Coefficient
(At Absorbance max):
32000
 
 
 
 
 
 
 
 
 
Structure
Structure
 
 
 
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