miRNA Cloning Products

miRNA Cloning

 

3' Cloning Linkers

 
ο miRNA isolationϴ Ŭδ׿ Դϴ. ̷ cloning linkers miRNA ligationϴ ۾
ʿմϴ. Adenlyation ؼ pre-activated Ŀ ϸ cloning efficiency ֽϴ. IDT BanI cloning site ϸ ddC 3'-end blockǰ 5'-end adenylyl activated Ready Made linker oligonucleotide մϴ. ̰ miRNA cloning̳ RNA ligase ϴ application ٷ ֽϴ
 
Linker-1 original modban sequence BanI restriction site ԵǾ ֽϴ.
Linker-2 AvaI StyI restriction sites ԵǾ ֽϴ.
Linker-3 EcoRI MspI restriction sites ԵǾ ֽϴ.
linker self ligation 3'-terminal dideoxy-C (ddc)base modification Ǿ ְ cloning ٷ ֵ lyophoilizedǾ ˴ϴ. ATP T4 RNA Ligase ̿Ͽ ligation mix Ͻô 󵵷  Ͻø ˴ϴ.
 
Modifications:  5'-Adenylated; fully activated and ready for use
                      3'-end blocked with a dideoxy-C base
Purification:      HPLC purified
Quality Control: Identity confirmed by ESI Mass Spectrometry
                       Tested for reactivity with RNA ligase
 
Certificate of Analysis for each linker available online here
 
Description
Sequence Part # Pricing
1 nm miRNA Cloning Linker 1 /5rApp/CTGTAGGCACCATCAAT/3ddC/ 11-04-02-08 ₩245,000
1 nm miRNA Cloning Linker 2 /5rApp/CACTCGGGCACCAAGGA/3ddC/ 11-04-02-09 ₩245,000
1 nm miRNA Cloning Linker 3 /5rApp/TTTAACCGCGAATTCCAG/3ddC/ 11-04-02-10 ₩245,000
5 nm miRNA Cloning Linker 1 /5rApp/CTGTAGGCACCATCAAT/3ddC/ 11-04-03-05 ₩833,000
5 nm miRNA Cloning Linker 2 /5rApp/CACTCGGGCACCAAGGA/3ddC/ 11-04-03-06 ₩833,000
5 nm miRNA Cloning Linker 3 /5rApp/TTTAACCGCGAATTCCAG/3ddC/ 11-04-03-07 ₩833,000
miRNA Cloning Linker 3-Pack – 3 Linkers, 1 nm each 11-02-06-01 ₩630,000
 
 

Custom Adenylation

 
T4 RNA Ligase single-strand nucleic acid sequence adenylationϱ ATP մϴ. ̷ activated adenylated-oligo ι° single-stranded sequence 3'-OH ˴ϴ. Pyrophosphate linkage adenylated oligonucleotides ATP Ȳ T4 RNA Ligase substrates ˴ϴ. IDT chemical adenylation method Ͽ ø custom adenylation Ͽ RNA-Ligase ֵ մϴ. T4 RNA Ligase adenylated DNA linker adenylated RNA linker ȿ ϱ IDT adenylated DNA oligos õմϴ. IDT circularization ϱ Ͽ adenylated oligo 3'-end blocking ʿմϴ. Blocking ؼ 3'-Spacer C3 /3SpC3/ or dideoxycytosine /3ddC/ ˴ϴ.
 
Scale Price Price
250 nm 4,500,000
1 m  4,500,000
5 m  6,000,000
10 m 11,250,000
 
 

5' M.R.S (Multiple Restriction Site) miRNA Cloning Linker

 
5' M.R.S Linker sequence  3'miRNA Cloning linker͵ ֵ Ǿϴ. 5' phosphate group
ϴ RNA 5' end linking ǵ ȭ Ǿϴ. 1mM ATP Ͽ T4 RNA Ligase ؼ ̷ϴ.
ȿ Ʈ ־ Ban1 (Linker 1), StyI and AvaI (Linker 2) and EcoRI (Linker 3) ֽϴ. Doubly linked RNA reverse transcription Ǹ 3' cloning linker شϴ ȿҰ 5' M.R.S Linker sequence ȣȯ  concatamerization Ŭδ Ͼ ˴ϴ. M.R.S Linker ִ ߰ ȿ Ʈ specific 3' linkers ġ ends for directional cloning ֽϴ. EcoRI BanI M.R.S Linker/Linker 3 digestion ϸ BanI 5' end EcoRI 3' ˴ϴ.
 
 

miSPIKE 21-mer Internal RNA Control

 
miSPIKE synthetic 21-mer RNA GenBank, miRBase, Ǵ RNAdb ִ  ˷ RNA sequence͵ match ʴ
ϴ. RNA oligonucleotide 5' phosphate ռ Ǿ denaturing acrylamide gel small RNA enrichment size marker 3' ligation control 5' ligation ϴ.
 
Sequence: 5'- rCrUrCrArGrGrArUrGrGrCrGrGrArGrCrGrGrUrCrU -3'
 
Description
Part # Pricing
100 pm miSPIKE Internal RNA Control 51-01-03-10 ₩35,000
 
 

piSPIKE 31-mer Internal RNA Control

 
piSPIKE synthetic 31-mer RNA miSPIKE 10base 5' phosphate ϴ.
 
Sequence: 5'- rCrUrCrArGrGrArUrGrGrCrGrGrArGrCrGrGrUrCrUrCrArCrUrGrArArCrGrU -3'
 
Description
Part # Pricing
100 pm piSPIKE Internal RNA Control 51-01-16-44 ₩49,000
 
 
 
 
 
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