xGen Exome Research Panel
CAT# PRODUCT SIZE STORAGE Һڰ ֹ
105611416 rxn xGen Exome Research Panel v1.016 rxn-α
105611596 rxn xGen Exome Research Panel v1.096 rxn-α
1080577xGen Hybridization and Wash Kit, 16 rxn16 rxn-α
1080584xGen Hybridization and Wash Kit, 96 rxn96 rxn-α
1077675xGen Library Amplification Primer Mix, 16 rxn16 rxn-α
1077676xGen Library Amplification Primer Mix, 96 rxn96 rxn-α
1077677xGen Library Amplification Primer Mix, 192 rxn192 rxn-α


xGen Exome Research Panel


Deep, uniform coverage of the human exome with best-in-class capture panel


The xGen Exome Research Panel v1.0 consists of 429,826 individually synthesized, and quality controlled xGen Lockdown Probes. The Exome Research Panel spans a 39 Mb target region (19,396 genes) of the human genome and covers 51 Mb of end-to-end tiled probe space. All probes in the panel are manufactured using GMP standards. Mass spectrometry and OD measurements are taken for each probe to ensure appropriate representation of the correctly manufactured probes in the pool.


  • Obtain consistent results with individually synthesized target capture probes manufactured to GMP standards
  • Achieve deep and uniform coverage even across difficult GC-rich regions
  • Integrate with common sequencing platforms


xGen Exome Research Panel v1.0

Product

Pricing

16 rxn xGen Exome Research Panel v1.0

₩8,640,000 KRW

96 rxn xGen Exome Research Panel v1.0

₩43,200,000 KRW


 


Exome sequencing is invaluable for sequencing only the coding regions of the human genome. With exons representing only 3% of the genome, it is critical to have an effective method of separating these regions from non-coding DNA to focus on potentially important mutations implicated in disease. The captured material must also be suitable for sequencing to a satisfactory depth of coverage for reliable detection of variant alleles.


The xGen Exome Research Panel consists of 5 biotin–modified oligonucleotide probes that are individually synthesized and individually analyzed by electrospray ionization mass spectrometry (ESI-MS) and OD measurement. The probes are then normalized before pooling to ensure that each probe is represented in the panel at the correct concentration. Probes that fail quality control are resynthesized. This rigorous manufacturing process gives the xGen Exome Research Panel a unique advantage over array-derived pools, in which missing or truncated probes cannot be identified before sequencing. Using IDT proprietary synthesis methods, even probes with high GC and AT content are appropriately represented in the panel.


To provide increased depth of coverage and enable high multiplexing of samples, the xGen Exome Research Panel targets only the coding sequences (CDS) of human coding genes in the RefSeq database.


 


The xGen Exome Research Panel v1.0 consists of 429,826 GMP compliant, individually synthesized, and quality controlled xGen Lockdown Probes spanning a 39 Mb target region of the human genome covered by 51 Mb of end-to-end tiled space.

Exome panel details »

The xGen AML Cancer Panel v1.0 comprises 11,743 xGen Lockdown Probes, spanning 1.19 Mb of the human genome, for targeted enrichment of >260 genes associated with the AML to provide more efficient detection of disease-causing mutations.

AML panel details »

The xGen Pan-Cancer Panel v1.5 consists of 7816 xGen Lockdown Probes, spanning 800 kb of the human genome, that capture 127 significantly mutated genes implicated across 12 tumor types for deeper sequencing coverage.

Cancer panel details »

The xGen Inherited Diseases Panel v1.0 consists of 116,355 xGen Lockdown Probes, spanning 11.1 Mb of the human genome, designed for targeted enrichment of genes and SNPs associated with inherited diseases.

Inherited diseases panel details »

The xGen Human ID Research Panel consists of 229 xGen Lockdown Probes for targeted enrichment of a definitive set of single nucleotide polymorphisms (SNPs) selected to identify a unique individual from a population of over 5 million biological samples.

Human ID panel details »

The xGen Human mtDNA Research Panel provides of 138 xGen Lockdown Probes for cost-effective and accurate detection of sequence variants in mitochondrial DNA.

Human mtDNA panel details »

Supplementing xGen Lockdown Panels

xGen Lockdown Panels can be customized by supplementing with additional probes. When supplementing an xGen Lockdown Panel, it is important to add the new probes at the same molar concentration as the probes in the original pool. The following table lists the molar concentration of each probe for a single hybrid capture reaction.

Panel Number of Probes fmol per Reaction
xGen Exome Research Panel v1.0  429,826 0.025 
xGen Acute Myeloid Leukemia Cancer Panel v1.0 11,743 0.255
xGen Pan-Cancer Panel v1.0 7,816 0.384
xGen Inherited Diseases Panel v1.0 116,355 0.026

For step-by-step instructions on how to supplement xGen Lockdown Panels with additional probes, contact contact our NGS technical support group at xgen@idtdna.com for the Probe Supplementation (Spike-In) Protocol.

ı
ıⰡ ϴ.
ı ۼ
gototop