xGen® Blocking Oligos
Improve on-target performance during targeted sequencing by reducing adapter-to-adapter hybridization
xGen Blocking Oligos bind to library adapter sequences to reduce off-target capture during library enrichment. Choose from convenient, optimized, predesigned mixes or build your own custom blocking oligo. xGen Universal Blockers are designed for use with Illumina indexed adapters. These ready-to-use mixes effectively block dual- or single-index adapters to significantly improve on-target performance.
Custom xGen Blocking Oligos are also available for other platforms, including Ion Torrent, and specialized applications.
Blocking oligos for Illumina Adapters
Universal blockers—TS Mix is compatible with ligation-based library prep techniques that use 6- and 8-base, single- and dual-indexing schemes. Universal blockers—10 bp TS Mix is compatible with ligation-based library prep kits that use 10 bp dual-indexing schemes. Universal blockers—NXT Mix is compatible with Illumina’s Nextera library kits with dual-indexing schemes.
For previously offered xGen Universal Blocking Oligos delivered separately for dual- and single-index adapters, click here to order or download the resuspension protocol. For information about future availability of these legacy products, please contact email@example.com.
All xGen Blocking Oligos are compatible with any xGen Lockdown® Panel or custom xGen Lockdown Probe set. Each oligo is individually synthesized and assessed by mass spectrometry for quality control.
xGen Universal Blockers
IDT currently offers three different ready-to-use, universal blocker reagents. xGen Universal Blockers—TS Mix blocks Illumina LT (p5, p7 – 6 nt and 8 nt) and HT (i5, i7) adapters and is compatible with ligation-based library prep kits, such as Illumina TruSeq kits. xGen Universal Blockers—10 bp TS Mix is compatible with ligation-based library prep kits that use 10 bp index barcodes. xGen Universal Blockers—NXT Mix contains adapter blockers that are optimized for use with Illumina Nextera library prep kits.
xGen Universal Blockers are offered in 16-, 96-, and 4 x 96-reaction formats for convenient experiment design and for use with xGen Lockdown Panels or xGen Lockdown Probe sets.
Custom xGen Blocking Oligos
Custom blocking oligos can be ordered for specialized applications or other sequencing platforms, and are offered with specified modifications. While we have identified the most commonly used modifications and services required for effective blocking in next generation sequencing, we understand that certain modifications or services may improve the performance of blocking oligo performance for your specific application.
For more information about available blockers, please contact our customer care team at firstname.lastname@example.org.