Electrophoresis system
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Quick Start Manual
After the agarose gel has
solidified, sample loading and electrophoresis can begin.
Agarose gels can be run in
many different types of electrophoresis buffers.
Nucleic acid agarose gel
electrophoresis is usually conducted with either Tirs-Acetate-EDTA(TAE) buffer
or Tris-Borate-EDTA(TBE) buffer. While TAE buffer provides faster
electrophoretic migration of linear DNA and better resolution of supercoiled
DNA, TBE buffers have a stronger buffering capacity for longer or higher voltage
electrophoresis runs.
1. Prepare sampels for gel
loading. Lading volume is dependent upon the type of comb used(i.e., well
thickness and length) and thickness of the gel.
2. When lading volume is
determined, add standard nucleic acid sample lading dye to a final 1X
concentration to make samples dense for underlaying into sampel
wells.
3. Load the samples into
the wells using standard pipets.
4. Place the lid on the
cell carefully. Do not distrub the samples. This system lids attach ot the base
in only one orientation. To attach the lid correctly, match the red and black
banana jacks on the lid with the red and black banana plugs of the
base.
5. Power requirements very
depending on gel thickness, length and concentration, and type of
electorphoresis buffer used.
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