IDT
IDT
The epigenome is regulated by three families of genes: proteins add, bind to, or remove DNA and histone modifications 1,2. These are commonly grouped as 'Writers,' 'Readers,' or 'Erasers,' respectively. The shRNA-mir epigenetics focused sets mirror these gene families and conveniently provide shRNA targeting epigenetics-related genes arrayed (in 96 well plates) or pooled for screening.
Based on the shERWOOD shRNA design algorithm, every shRNA is expected to knockdown the targeted gene by > 70%. This consistency increases confidence in results providing a distinct advantage over other shRNA libraries. Click here to learn more about the shERWOOD algorithm. Pooled screening libraries – Screen the epigenome without robotics or liquid handling
The pooled screening library provides complete coverage across all epigenetics-related genes for human or mouse. Gene coverage:
Viral particle format includes:
Plasmid DNA format includes:
Screening capacity:
Arrayed focused sets: The complete epigenetics-focused shRNA sets includes genes curated by Dr. Greg Hannon and colleagues at Cold Spring Harbor Laboratory and review literature. These can be further broken down by activity or functional domain. Smaller arrayed focused sets are also available targeting genes based on a specific activity or functional domain. See table below. (All focused sets are available in pooled format upon request.)
Smaller libraries, grouped by function, are also available in as arrayed libraries:
Epigenetics-related genes grouped by function:
References1. Epigenetic protein families: a new frontier for drug discovery; Arrowsmith et al., Nature Reviews Drug Discovery 11, 384-400 (May 2012)2. Unpublished data from Dr. Greg Hannon, Cold Spring Harbor Laboratory. 3. Epigenetic modifications as therapeutic targets, Kelly, et al., Nature Biotechnology 28, 1069–1078, (2010) Epigenetics-related human genesThe table below highlights each gene and the type of products available. Clicking on the gene symbol automatically submits that gene to the FETCH my gene ™ search tool. View the results for all availble products by selecting a tab on the FETCH my gene results page. For individual products click the link shown in each column.
리스트 확인 페이지Mouse Genes in the Epigenetics Focused SetsThe combined focused sets for mouse Epigenetics includes 393 genes. The table below highlights each gene and the type of products available. Clicking on the gene symbol automatically submits that gene to the FETCH my gene ™ search tool. View the results for all availble products by selecting a tab on the FETCH my gene results page. For individual products click the link shown in each column.
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MLP-Retroviral-shRNA-mir-Technical-Manual.pdf (893,729kb) |
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