MGC premier Expression-Ready cDNA clones
for Human, Mouse, Rat and Bovine genomes
Expression-Ready vector options combined with the highest quality full-length cDNA content provide the fastest path to a successful experiment.  All MGC premier Expression-Ready cDNA clones come with a 100% guarantee* for sequence integrity and expression of the cloned gene.




Choice of expression vectors

Select from three robust expression vectors with a choice of mammalian selection markers:
  • CMV: CMV promoter with no selectable marker (pCS6)
  • CMV-Neo:  CMV promoter with a neomycin selectable marker (pTCN)
  • CMV-Puro: CMV promoter with a puromycin selectable marker (pTCP)
These vectors provide a simple and effective expression system:
  • Robust expression from the CMV promoter
  • Choice of the commonly used selection markers
  • Guaranteed* expression
 Use the Fetch my Gene¢â search tool to find and purchase cDNA clones representing your gene of interest

*The MGC premier Expression-ready cDNA vectors have been validated for expression. Expression of your target gene is guaranteed (as compared to control gene expression in your target cell line).  For assistance please contact support@transomic.com.

 

Product Specification Sheets


TCH3001 - MGC premier Human cDNA set - Cell Cycle (pCMV)
TCH3003 - MGC premier Human cDNA set - GPCR (pCMV)
TCH3005 - MGC premier Human cDNA set - Ion Channel (pCMV)
TCH3007 - MGC premier Human cDNA set - Nuclear Receptor (pCMV)
TCH3002 - MGC premier Human cDNA set - Ubiquitin (pCMV)
TCH3004 - MGC premier Human cDNA set - Transcription Factor (pCMV)
TCH3006 - MGC premier Human cDNA set - Kinases (pCMV)
TCH3009 - MGC premier Human cDNA set - Phosphatases (pCMV)

MGC premier Expression-Ready cDNA clones

 

Visual validation of pTCP and pTCN expression. TurboGFP was inserted into each expression vector in place of the cDNA.  HEK293T cells were transfected with pTCN expressing tGFP (A), pTCP expressing TurboGFP (B) and a previously validated TurboGFP expression vector (C).

pTCP and pTCN Validation

pTCN and pTCP expression was visually assessed using the fluorescent marker TurboGFP.  TurboGFP was inserted into each expression vector in place of the cDNA.  Cells were treated following the OMNIfect transfection protocol and fluorescence was visualized after 48 hrs.  Expression was compared to a known TurboGFP expression vector.  Representative fields from the biological replicates of each vector are shown in adjacent figure.  All vectors show robust expression.
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