Alpha-Select Silver Efficiency
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-85026Competent Cells, Silver Effici10 X 200¥ìLat -80C¡Æ-·Î±×ÀÎ
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¥á-Select Silver Competent Cells are ideal for difficult clone construction and blunt-end ligation.
 
 
 
 
 

 
 

Features & Benefits

Reduced recombination of cloned DNA -designed to accommodates larger plasmids
High efficiency - available in ¡Ã108 cfu/¥ìg, perfect for difficult clones
Easy screening -with blue/white color selection
Convenient size -small aliquots reduce waste

Applications

Transformation of large plasmids into bacterial cells 
Generating clones Blue/white color screening  

Description

¥á-Select Silver Competent Cells are directly comparable to DH5¥á cells. They contain a lacZ marker that provides ¥á-complementation of the ©¬-galactosidase gene for blue/white color screening and are ideal for difficult clone construction and blunt end ligation using plasmid-derived vectors.
¥á-Select Silver Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control.

Components

Reagent
BIO-85026
Chemically Competent Cells
10 x 200¥ìl
pUC19 control vector (10pg/¥ìl)
100¥ìl

Notes

Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA ¥Ä(lacZYA-argF)U169 ¥Õ80lacZ¥ÄM15 ¥ë- 

Storage & Stability

All components should be stored at -80¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.

Shipping conditions

Shipped on dry/blue ice
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