¥á-Select Silver Competent Cells are ideal for difficult clone construction and blunt-end ligation.
Features & BenefitsReduced recombination of cloned DNA -designed to accommodates larger plasmids
High efficiency - available in ¡Ã108 cfu/¥ìg, perfect for difficult clones
Easy screening -with blue/white color selection
Convenient size -small aliquots reduce waste
ApplicationsTransformation of large plasmids into bacterial cellsGenerating clones Blue/white color screening
Description¥á-Select Silver Competent Cells are directly comparable to DH5¥á cells. They contain a lacZ marker that provides ¥á-complementation of the ©¬-galactosidase gene for blue/white color screening and are ideal for difficult clone construction and blunt end ligation using plasmid-derived vectors.
¥á-Select Silver Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
pUC19 DNA is also provided as a positive control. Components
NotesGenotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA ¥Ä(lacZYA-argF)U169 ¥Õ80lacZ¥ÄM15 ¥ë- Storage & StabilityAll components should be stored at -80¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label. Shipping conditionsShipped on dry/blue ice |
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