Uracil DNA Glycosylase
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-27044Uracil DNA Glycosylase500U-20'C-·Î±×ÀÎ
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  Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides.
 




 

Features & Benefits

Removes uracils fron DNA - causes fragmentation of DNA and stops amplification in a PCR
Easy-to-use - novel, sensitive mutant is irreversibly inactive after heating
 

Applications

Control of carry-over contamination in PCR As a probe for protein-DNA interaction studies Cloning of PCR products
 

Description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).
UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM).
Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.
Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.

Components

Reagent
500 Units
Uracil DNA Glycosylase
500¥ìl
10x Reaction Buffer
1.2ml

Concentration


1u/¥ìl

Storage & Stability

All components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided.
When stored under the recommended conditions and handled correctly, full activity of the reagents is retained until the expiry date indicated on the outer box label.
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