Real-time PCRÀº °¡Àå °·ÂÇÏ°í ¹Î°¨ÇÑ À¯ÀüÀÚ ºÐ¼® ±â¼ú Áß Çϳª·Î ¸ðµç ½ÇÇè½Ç¿¡¼ ±âÁØÀÌ µÇ°í ÀÖ½À´Ï´Ù. Ưº°È÷ ÀûÀº ¾çÀÇ nucleic acid¸¦ ÁõÆøÇÏ°í Á¤·®Çϴµ¥ ÀÖ¾î¼ÀÇ ±× È¿À²¼ºÀº ¼¼Æ÷³» mRNA¿Í miRNAÀÇ Á¤·®, biomarker ¹ß°ß°ú validation, Microarraying, ¹Ì»ý¹° Á¤·®, ¾ÏÀ§Çèµµ Æò°¡, gene dosage °áÁ¤, ¹ýÀÇÇÐÀû Á¶»ç¿¡¼ ¸Å¿ì ÀûÀº »ùÇÃÀÇ °ËÃâ µî ´Ù¾çÇÑ Àû¿ëºÐ¾ßÀÇ ÃâÇöÀ» À¯µµÇÏ¿´½À´Ï´Ù. Real-time PCR»ç¿ëÀÇ Áõ°¡´Â Àüü ½ÇÇè ½Ã°£À» ÁÙÀÏ ¼ö high throughput°ú ´õ ºü¸¥ assay¿¡ ´ëÇÑ ¿ä±¸ÀÇ Áõ°¡¸¦ °¡Á®¿Ô½À´Ï´Ù. ÀÌ·¯ÇÑ ¿ä±¸¿¡ ±â°èÀÇ °³¼±°ú ´õ ºü¸¥ È¿¼Ò(Á¤È®µµÀÇ Èñ»ý ¾øÀÌ)ÀÇ »ç¿ë°ú PCR ½ºÅÜÀ» ÁÙÀÌ´Â ¹æ¹ý µîÀÌ ÀÌ·ç¾îÁ³½À´Ï´Ù. »õ·Î¿î SensiFAST¢â Kits´Â ÀÌ·¯ÇÑ µµÀüÀ» ¹Þ¾ÆµéÀ̱â À§ÇØ °³¹ßµÇ¾î antibody±â¹Ý hot-start enzyme°ú ¸Å¿ì ºü¸¥ ÇÕ¼º ¼Óµµ¸¦ Á¦°øÇÏ¿© fast cycling Á¶°ÇÀÌ °¡´ÉÇÏ°Ô ÇÏ¿´½À´Ï´Ù. SensiFAST¢â ±¸¼ºÀº ±âÁ¸ standard qPCRÀÇ ¼º´É, ½Å·Ú¼º, ³ôÀº Àç»ý¼ºÀº À¯ÁöÇÏ¸é¼ °¡Àå ºü¸¥ cycling timeÀ» Á¦°øÇÕ´Ï´Ù. SensiFAST¢â´Â ¸ðµç real-time ±â°è¿Í »ç¿ëÀÌ °¡´ÉÇϸç Ưº°È÷ Â÷¼¼´ë fast cycler¿Í µµ »ç¿ë °¡´ÉÇÕ´Ï´Ù.
Features and Benefits• Á¤È®ÇÑ Á¤·® - hot-start±â´ÉÀ¸·Î primer-dimerÇü¼ºÀ» ÁÙÀÓ
• ¹Î°¨¼º - ÀûÀº ¾çÀÇ targetÀ¸·Î ÁõÆø °¡´É • ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë • ȣȯ¼º - fast cycling PCRÀ» Æ÷ÇÔÇÑ ¸ðµç real-time PCR±â°è¿Í ȣȯ • Protocol - fast qPCR °ú standard qPCR protocol ¸ðµÎ °¡´É Instrument CompatibilityABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne¢â, StepOne¢â Plus (See product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.
DescriptionSensiFAST SYBR Kits´Â ºü¸£°í Àç»ý °¡´É °¡´ÉÇÑ real-time PCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. »ç¿ëÇϱâ ÆíÇϵµ·Ï SensiFAST SYBR Kit´Â 2X matermix typeÀ¸·Î Á¦°øµÇ¸ç real-time PCR¿¡ ÇÊ¿äÇÑ ¸ðµç SYBR Green I Dye, dNTPs, stabilisers¿Í enhancers±îÁö ¸ðµÎ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î reverse
transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. 18~19pageÀÇ compatibility table ¿¡ ÀÖ´Â ±â°è¿¡¼ SYBR Green-based real-time PCR »ç¿ë¿¡ ÃÖÀûȵǾú½À´Ï´Ù. ÀÌ Å°Æ®´Â ¶ÇÇÑ ROX¸¦ »ç¿ëÇÏÁö ¾Ê´Â ´ÙÀ½ÀÇ ±â°è¿Íµµ ȣȯ °¡´ÉÇÕ´Ï´Ù. Qiagen(Corbett), Rotor-Gene 6000, Bio-Rad CFX 96, Roche LightCylcer 480) SensiFAST SYBR Hi-ROX¿Í Lo-ROX kitÀº ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. SensiFAST SYBR & Fluorescein Kit´Â ¼±Åà »ç¿ëÀ» À§ÇØ premixed FluoresceinÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. VolumeBIO-92002: 200 x 20¥ìl Reactions: 2 x 1ml
BIO-92005: 500 x 20¥ìl Reactions: 5 x 1ml BIO-92020: 2000 x 20¥ìl Reactions: 20 x 1ml Kit SizeThe kit size is based on a 20¥ìl final reaction volume.
Storage Conditions12 months at -20¡ÆC. Avoid exposure of the SYBR¢ç Green I to light.
Product CitationsPresnell, S.R., et al. J. Imm. 188(9), 4394-4404 (2012).
Gocek, E., et al. J. Steroid Biochem Mol Biol. e28972 (2012). NotesSensiFAST is a trademark of Bioline Reagents Ltd.
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