E. coli strain GB05-dir


* 2019  ÀÌÈÄ Ãë±Þ ¾ÈÇÔ*

Features

  • Direct cloning of DNA fragments into a linear plasmid backbone
  • E. coli strain harboring an arabinose inducible ET¥ãA operon (full length recE, recT, red¥ã, and recA) at the ybcC locus

Applications

  • Cloning of PCR products
  • Cloning of linear DNA fragments from digested genomic DNA (Fu et al. 2012)

Description

Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from conventional recombineering mediated by Red¥á¥â from lambda phage. E. coli strain GB05-dir enables direct cloning of DNA fragments without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.

Contents

  • Glycerol stock of direct cloning-proficient E. coli strain GB05-dir
  • Linear control plasmid backbone (ColE1 origin of replication and an ampicillin resistance marker gene)
  • Linear control insert (PCR product)
  • Protocol

K008-Manual-web.pdf (3,126,796kb)
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