E. coli strain GB08-red

* 2019  ÀÌÈÄ Ãë±Þ ¾ÈÇÔ*

Recombineering-proficient E. coli strain for linear plus circular homologous recombination
 

Features

  • Recombineering-proficient E. coli strain
  • E. coli strain harboring an arabinose inducible ¥ã¥â¥áA operon (red¥ã, red¥â, red¥á and recA) at the ybcC locus

Applications

  • All types of "linear plus circular" homologous recombination experiments, e.g. plasmid modifications

Description

E. coli strain GB08-red enables recombineering of plasmids and BACs without the necessity to maintain an expression plasmid in the cells since the necessary genes are located on the chromosome.

Contents

  • Glycerol stock of recombineering-proficient E. coli strain GB08-red
  • Linear control insert (PGK-gb2-neo PCR product)
  • Circular control plasmid (high copy plasmid containing 15kb of the mouse Hoxa11 gene)
  • Protocol

 
 
K009-Manual-web.pdf (3,029,476kb)
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