SensiFAST¢â SYBR Lo-ROX Kit
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
BIO-94005SensiFAST SYBR Lo-Rox Kit500 RXNSat -20C¡Æ-·Î±×ÀÎ
BIO-94020SensiFAST SYBR Lo-Rox Kit2000 RXNSat -20C¡Æ-·Î±×ÀÎ
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 Real-time PCRÀº °¡Àå °­·ÂÇÏ°í ¹Î°¨ÇÑ À¯ÀüÀÚ ºÐ¼® ±â¼ú Áß Çϳª·Î ¸ðµç ½ÇÇè½Ç¿¡¼­ ±âÁØÀÌ µÇ°í ÀÖ½À´Ï´Ù. Ưº°È÷ ÀûÀº ¾çÀÇ nucleic acid¸¦ ÁõÆøÇÏ°í Á¤·®Çϴµ¥ À־ÀÇ ±× È¿À²¼ºÀº ¼¼Æ÷³» mRNA¿Í miRNAÀÇ Á¤·®, biomarker ¹ß°ß°ú validation, Microarraying, ¹Ì»ý¹° Á¤·®, ¾ÏÀ§Çèµµ Æò°¡, gene dosage °áÁ¤, ¹ýÀÇÇÐÀû Á¶»ç¿¡¼­ ¸Å¿ì ÀûÀº »ùÇÃÀÇ °ËÃâ µî ´Ù¾çÇÑ Àû¿ëºÐ¾ßÀÇ ÃâÇöÀ» À¯µµÇÏ¿´½À´Ï´Ù. Real-time PCR»ç¿ëÀÇ Áõ°¡´Â Àüü ½ÇÇè ½Ã°£À» ÁÙÀÏ ¼ö high throughput°ú ´õ ºü¸¥ assay¿¡ ´ëÇÑ ¿ä±¸ÀÇ Áõ°¡¸¦ °¡Á®¿Ô½À´Ï´Ù. ÀÌ·¯ÇÑ ¿ä±¸¿¡ ±â°èÀÇ °³¼±°ú ´õ ºü¸¥ È¿¼Ò(Á¤È®µµÀÇ Èñ»ý ¾øÀÌ)ÀÇ »ç¿ë°ú PCR ½ºÅÜÀ» ÁÙÀÌ´Â ¹æ¹ý µîÀÌ ÀÌ·ç¾îÁ³½À´Ï´Ù. »õ·Î¿î SensiFAST¢â Kits´Â ÀÌ·¯ÇÑ µµÀüÀ» ¹Þ¾ÆµéÀ̱â À§ÇØ °³¹ßµÇ¾î antibody±â¹Ý hot-start enzyme°ú ¸Å¿ì ºü¸¥ ÇÕ¼º ¼Óµµ¸¦ Á¦°øÇÏ¿© fast cycling Á¶°ÇÀÌ °¡´ÉÇÏ°Ô ÇÏ¿´½À´Ï´Ù. SensiFAST¢â ±¸¼ºÀº ±âÁ¸ standard qPCRÀÇ ¼º´É, ½Å·Ú¼º, ³ôÀº Àç»ý¼ºÀº À¯ÁöÇϸ鼭 °¡Àå ºü¸¥ cycling timeÀ» Á¦°øÇÕ´Ï´Ù. SensiFAST¢â´Â ¸ðµç real-time ±â°è¿Í »ç¿ëÀÌ °¡´ÉÇϸç Ưº°È÷ Â÷¼¼´ë fast cycler¿Í µµ »ç¿ë °¡´ÉÇÕ´Ï´Ù.
 
 

Features & Benefits

• Á¤È®ÇÑ Á¤·® - hot-start±â´ÉÀ¸·Î primer-dimerÇü¼ºÀ» ÁÙÀÓ
• ¹Î°¨¼º - ÀûÀº ¾çÀÇ targetÀ¸·Î ÁõÆø °¡´É
• ºü¸§ - ÃÖ°íÀÇ Æ¯À̼º°ú ¹Î°¨µµ¸¦ À§ÇØ µ¶Æ¯ÇÑ ¹öÆÛ »ç¿ë
• ȣȯ¼º - fast cycling PCRÀ» Æ÷ÇÔÇÑ ¸ðµç real-time PCR±â°è¿Í ȣȯ
• Protocol - fast qPCR °ú standard qPCR protocol ¸ðµÎ °¡´É

Instrument Compatibility

ABI 7500, 7500 FAST, ViiA7¢â, Stratagene (Agilent) Mx4000¢â, Mx3000P¢â, Mx3005P¢â (See product selection table), as well as other instruments that do not require the use of a passive reference. 
 

Description

SensiFAST SYBR Kits´Â ºü¸£°í Àç»ý °¡´É °¡´ÉÇÑ real-time PCRÀ» À§ÇØ °³¹ßµÇ¾úÀ¸¸ç ÀϹÝÀûÀ¸·Î »ç¿ëµÇ°í ÀÖ´Â real-time ±â°è¿¡¼­ °ËÁõÀÌ µÇ¾ú½À´Ï´Ù. ÃÖ½ÅÀÇ buffer Á¶¼º°ú enhancer ±×¸®°í antibody ±â¹Ý hot-start DNA PolymeraseÀÇ Á¶ÇÕÀº SensiFAST SYBR kit°¡ ºü¸£°í ³ôÀº Àç»ýÀ²°ú ÃÖ°í·Î ¹Î°¨ÇÑ real-time PCRÀÌ °¡´ÉÇϵµ·Ï ÇÕ´Ï´Ù. »ç¿ëÇϱâ ÆíÇϵµ·Ï SensiFAST SYBR Kit´Â 2X matermix typeÀ¸·Î Á¦°øµÇ¸ç real-time PCR¿¡ ÇÊ¿äÇÑ ¸ðµç SYBR Green I Dye, dNTPs, stabilisers¿Í enhancers±îÁö ¸ðµÎ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. ÀÌ kit´Â DNA template¿¡ ÀûÇÕÇÏ°í RNA templateÀº Bioline Tetro cDNA Synthesis Kit(BIO-65042)·Î reverse
transcriptionÇÑ ÈÄ »ç¿ë °¡´ÉÇÕ´Ï´Ù. 18~19pageÀÇ compatibility table ¿¡ ÀÖ´Â ±â°è¿¡¼­ SYBR Green-based real-time PCR »ç¿ë¿¡ ÃÖÀûÈ­µÇ¾ú½À´Ï´Ù. ÀÌ Å°Æ®´Â ¶ÇÇÑ ROX¸¦ »ç¿ëÇÏÁö ¾Ê´Â ´ÙÀ½ÀÇ ±â°è¿Íµµ ȣȯ °¡´ÉÇÕ´Ï´Ù. Qiagen(Corbett), Rotor-Gene 6000, Bio-Rad CFX 96, Roche LightCylcer 480) SensiFAST SYBR Hi-ROX¿Í Lo-ROX kitÀº ¼±Åûç¿ëÀ» À§ÇØ premixed ROX°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. SensiFAST SYBR & Fluorescein Kit´Â ¼±Åà »ç¿ëÀ» À§ÇØ premixed FluoresceinÀÌ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.

Notes

SensiFAST is a trademark of Bioline Reagents Ltd.

Components

Reagent
200 x 20µl Reactions
500 x 20µl Reactions
2000 x 20µl Reactions
SensiFAST SYBR¢ç Lo-ROX mix (2x)
2 x 1ml
5 x 1ml
20 x 1ml
 

Storage & Stability

All kit components should be stored at -20¡ÆC upon receipt for optimum stability. Repeated freeze/thaw cycles should be avoided. Avoid exposure of the SYBR¢ç Green I to light.
When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label.
 

Shipping conditions

Shipped on dry/blue ice.
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SensiFAST¢â SYBR Hi-ROX Kit
SensiFAST¢â SYBR & Fluorescein Kit
SensiFAST¢â SYBR No-ROX Kit
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