SeeNA¢â Nucleice Acid Stains
CAT# PRODUCT SIZE STORAGE ¼ÒºñÀÚ°¡ ÁÖ¹®
10300SeeNA¢â Nucleice Acid Stains1 ml-20¡ÆC-·Î±×ÀÎ
10301SeeNA¢â Nucleice Acid Stains5 ml-20¡ÆC-·Î±×ÀÎ
10311SeeNA¢â II Nucleice Acid Stains5 mlRT-·Î±×ÀÎ
10310SeeNA¢â II Nucleice Acid Stains1 mlRT-·Î±×ÀÎ

SeeNA¢â and SeeNA¢â II Nucleic Acid Stains

SeeNA¢â Ư¡ Nucleic acid stain solution series´Â UltrasensitiveÇϸ鼭µµ Non-toxicÇÑ ¹°Áú·Î½á DNA/RNA¸¦ È¿°úÀûÀ¸·Î ¿°»öÇØ ÁÖ´Â ½Ã¾àÀÔ´Ï´Ù. ¹ß¾Ï¹°ÁúÀÎ EtBrÀ» ´ëüÇÏ¿© ´õ¿í ¾ÈÀüÇÏ°Ô ÇÙ»ê Àü±â¿µµ¿À» ÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÈ SeeNA¢â Series´Â LED illuminator ¶Ç´Â UV transilluminator ¸ðµÎ ¿¡¼­ °üÂûÀÌ °¡´ÉÇÑ ¸Å¿ì Æí¸®ÇÑ Á¦Ç°ÀÔ´Ï´Ù.
 
¡¡ SeeNA¢â Ư¡ SeeNA¢â II Ư¡
Applications Àü±â ¿µµ¿ Àü¿¡ »ùÇÿ¡ Á÷Á¢ ¿°»ö °¡´É Àü±â ¿µµ¿ Àü¿¡ »ùÇÿ¡ Á÷Á¢ ¿°»ö °¡´ÉÇϳª ±ÇÀåÇÏÁö ¾ÊÀ½
EtBr ó·³ ³ìÀÎ Agarose Gel¿¡ »ç¿ë ¾ÈµÊ  EtBr ó·³ ³ìÀÎ Agarose Gel¿¡ »ç¿ë °¡´É
DNA/ RNA ¿°»ö °¡´É DNA/ RNA ¿°»ö °¡´É
º¸°ü ¿Âµµ -20 ¡É in dark »ó¿Â in dark
»ç¿ë Àåºñ UV transilluminator UV transilluminator
LED illuminator  LED illuminator 
¿ë·® 1ml/ 5ml 1ml/ 5ml
»ç¿ë ¹æ¹ý 1/10 ·Î Èñ¼® ÈÄ 5~7 ul DNA/RNA »ùÇÿ¡ 1ul¾¿ »ç¿ë Agarose Gel 100ml ´ç   5ul ÀÇ SeeNA¢â II »ç¿ë
»ç¿ë¾ç 1ml SeeNA¢â - 10,000 »ùÇà »ç¿ë °¡´É 1ml SeeNA¢â II - 20LÀÇ Agarose gel »ç¿ë °¡´É
Toxicity ¹«µ¶¼º   ¹«µ¶¼º
 
   
 
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1% agarose in 0.5X TBE Voltage of 100V, 100bp(1~3 lane) and 1kb ladder (4~6 lane)
 

Overview

SeeNATM and SeeNATM II Nucleic Acid Stains developed by Mbiotech are a kind of new generation of fluorescent nucleic acid gel stains designed to replace the highly toxic ethidium bromide (EtBr). SeeNATM Nucleic Acid Stains is nontoxic and highly sensitive than EtBr. Gels can be visualized in UV transilluminator or Visible Light Transilluminator.
 

Notes :

1. Do not run gels over 2 hrs. Or smeared bands appeared because SeeNATM Nucleic Acid Stain will dissociate from DNA/RNA.
2. SeeNATM Nucleic Acid Stain can dissociate from nucleic acids in ethanol.
3. Please stain nucleic acid in the gel or after electrophoresis to check the exact molecular weight of fragments when compared with molecular weight markers.
4. Please stain DNA with DNA stain and RNA with RNA stain.
5. Pelase use EP tubes and other plastic wares in SeeNATM Nucleic Acid Stain storage, dilution and staining. SeeNATM Nucleic Acid Stain can bind to glassware.
 

Features

* Safety: nontoxic and noncarcinogenic.
* Ultrasensitivity: allows the visualization of as little as 20pg dsDNA, around 5-10 times more sensitive than EtBr in UV transilluminator and 8-20 times more sensitive than EtBr in Visible Light Transilluminator.
* Convenience: no need to rinse or wash gels. Add stain before load samples. Visualize gels in SeeNA¢â LED Transilluminator or in Visible Light Transilluminator to avoid UV damage on DNA/RNA. UV transilluminator also is available for broad usage.
* Wide range: suitable for agarose gel or PAGE
* No effect on downstream experiments: RT, PCR, enzyme digestion, and ligation.
* Strong signal and no background.
* Low cost: 1ml SeeNATM Nucleic Acid Stain is sufficient to load 10,000 samples.

Protocols

Protocol 1: Stain nucleic acid in electrophoresis (add stain in the gel for SeeNA¢â II)

1. Make gels: Add 5µl SeeNATM II Nucleic Acid Stain per 100ml gel when cool down to 60¡É. Usually, 1ml SeeNATM II Nucleic Acid Stain is sufficient for making 200 gels (total 20 L).
2. Run gels based on the routine method.
3. Visualize gels in UV transilluminator. Or in LED illuminator to avoid UV damage on DNA/RNA.

Protocol 2: Stain nucleic acid before electrophoresis (add stain in the samples for SeeNA¢â)

1. Prepare working solution: Dilute 100 µl SeeNATM Stain with 1ml running buffer TBE or TAE. This solution is stable up to one month at 4¡É.
2. Make gels: based on the routine method. Do not add any DNA/RNA stain in the gel.
3. Stain Nucleic Acid: Add 1µl SeeNATM Nucleic Acid Stain working solution to 10µ mixture of sample and loading buffer, let it stay at RT for 3-5min for stain binding to nucleic acid completely. Normally, 1µl working solution is enough for one sample loading, and 1ml SeeNATM Nucleic Acid Stain is enough to load 10,000 samples.
4. Stain markers: Mix 5µL Marker and 1µL SeeNATM Nucleic Acid Stain working solution thoroughly, let it stay at RT for 5min to let SeeNATM Stain and DNA/RNA binding completely.
5. Load samples and run gels.
6. Visualize gels in UV transilluminator. Or in LED illuminator to avoid UV damage on DNA/RNA.
Usually, 1µl stain is enough to stain one sample. That is 1ml SeeNATM Stain is enough to load 10,000 samples. However, big fragments (>2kb) will move slowly when bind to the stain. So, please stain DNA after electrophoresis or add stain in gels to measure molecular weight exactly.

Protocol 3. Stain Nucleic Aicd after electrophoresis (add stain in gel staining solution)

 
1. Make gels: Do not add any nucleic acid stain when make gels.
2. Prepare SeeNATM Nucleic Acid Stain: dilute SeeNATM Nucleic Acid Stain with TAE or TBE on ratio 1:1000. Stain gels in the dark for 10-30 min. Staining time depends on gel concentration and tinkness. PAGE can be stained directly on the glass. Let staining solution cover PAGE gels for 30 min. Please use glassware to store staining solution or silicified glassware because stain will absorb on the glass.
3. Visualize gels in UV transilluminator. Or in SeeNATM LED illuminator to avoid UV damage on DNA/RNA.
 
10300-2013.pdf (257,365kb)
10310-2013.pdf (225,285kb)
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