IDT
IDT
DescriptionRibonuclease inhibitor effectively inhibits ribonuclease A type(ribonuclease A, B and C) enzyme activity by binding non-covalently to RNase molecules at a 1:1 ratio. It does not inhibit RNA polymerase, DNA polymerase, RNase H, or RNase T1, and is therefore ideal for use in RT-PCR reactions, in vitro tranlscription, or DNA library construction. Mbiotech RNase Inhibitor is a highly purified, nuclease-free preparation. Each lot is rigorously tested and is guaranteed free of non-specific enodnuclease, exonuclease, and RNase activity.
SourceE.coli strain carrying the gene of Ribonuclease Inhibitor. RNase A is not involved in the purification process.
Applications● Protection of mRNA in cDNA synthesis.
● Improvement of protein translation performance. ● Increased yields and activity of polysomes. ● Improvement of in vitro virus replication. ● Improvement of RNA translation in homologous systems. Unit definitionOne unit is defined as the amount of ribonuclease inhibitor required to inhibit by 50% activity of 5ng of ribonuclease A. Activity is measured by the inhibition of hydrolysis of cytidine 2’3’-cyclic monophosphate by ribonuclease A, where 200 units of ribonuclease inhibitor produces 50% inhibition of 1ug of ribonuclease A.
Standard unit assay conditionsActivity is measured by the inhibition of hydrolysis of cytidine 2',3'-cyclic monophosphate by ribonuclease A, where 200 units of ribonuclease inhibitor produces 50% inhibition of 1μg of ribonuclease A.
Concentration40 units/μl.
Strorage conditionAvoid multiple freeze/thaw cycles and frequent temperature changes. These fluctuations can greatly affect the product stability.
-Long term(Infrequent use:1-2 times/month):-70°C. -Short term(Daily/Weekly use):-20°C If stored for long periods at -20°C or opened frequently during storage, addition of DTT to 5mM final concentration into the reaction mixture is recommended to maintain optimal activity.
Note: Ribonuclease inhibitor is active over a broad pH range but requires a minimum of 1mM DTT to maintain activity. 19701-2014.pdf (136,438kb) |
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