*2019³â ÀÌÈÄ Ãë±Þ ÇÏÁö ¾ÊÀ½DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus
DNA Polymerase I Large (Klenow) Fragment, Exonuclease Minus (Klenow Fragment, Exo-) is a DNA-dependent DNA polymerase which catalyzes the 5¢¥¡æ3¢¥synthesis of DNA and lacks both the 5¢¥¡æ3¢¥ and the 3¢¥¡æ5¢¥ exonuclease activities.
Source:Purified from a strain of E. coli that carries a Klenow Fragment, Exo- expression plasmid.
Applications:
Heat inactivation: 70¡ÆC for 15 minutes
Storage: Enzyme and buffer should be stored at -20¡ÆC. Enzyme is stable for 12 months if handled properly.
Additional Information:
References:
1. Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.7-3.5.10. 2. Sanger, F. et al. (1977) Proc. Natl. Acad. Sci. USA, 74, 5463-5467. MSDS.pdf (118,131kb) |
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