*2019년 이후 취급 하지 않음
E. cloni® 10G and 10GF' Chemically Competent Cells
- Direct replacements for standard cloning strains (e.g., DH5α™, DH10B™, JM109, TOP10, XL1-Blue, etc.)
- Optimized genetics for high yields: phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.
- Available in a range of transformation efficiencies (1 × 106 to 1 x 109cfu/µg).
- Convenient packaging options.
Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5α™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. These cells provide solutions for a wide range of applications at economical prices.
Choice of strain:
- E. cloni 10G Competent Cells. Routine cloning, subcloning, and plasmid isolation with or without blue/white screening.
- E. cloni 10GF′ Competent Cells. Contain the F′ plasmid for infection with M13 to produce ssDNA
Choice of efficiency:
- E. cloni 10G Chemically Competent Cells (> 1 × 109 cfu/µg pUC DNA; (> 1 × 108 in 96-well plates). Highly efficient competent cells for routine cloning applications. Available in one transformation per tube (SOLOs) or two transformations per tube (DUOs). Also available in 96-well plates (which can be divided into 24-well seqments) for higher throughput.
- E. cloni 10GF´ Chemically Competent Cells (> 5 × 108 cfu/µg pUC DNA). An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.
- E. cloni 10G Chemically Competent Cells, Subcloning Grade (> 1 × 106 cfu/µg pUC DNA). The best value available anywhere for simple cloning and plasmid propagation.
Table 1. E. cloni Competent Cells for Cloning
| E. cloni Cell Lines |
Transformation Efficiency
(cfu/µg pUC DNA) |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
10G Chemically Competent |
>1 × 109 |
YES |
NO |
YES: without IPTG induction |
|
10G Chemically Competent Subcloning Grade |
>1 × 106 |
YES |
NO |
YES: without IPTG induction |
|
10GF´ Chemically Competent |
>5 × 108 |
YES |
NO |
YES: IPTG induction required |
Genotypes
- E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697 galU galK rpsL nupG λ- tonA
- E. cloni 10GF′: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA
Genotypes
E. cloni 10G: F- mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara,leu)7697galU galK rpsL nupG λ- tonA (StrR)
E. cloni 10GF´: [F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)
IDT