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CJ236 Electrocompetent Cells

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis

Sole source of highly efficient Electrocompetent cells (1 × 10⁹ cfu/µg)
ung- and dut- mutations to generate DNA with some uracil residues
Ideal genotype and efficiency for Kunkel directed mutagenesis method¹.

¹ Kunkel T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. 52:488-492.

Lucigen is the sole provider of high-efficiency electrocompetent CJ236 cells. This cell line was created by adding the pCJ105 plasmid to BW313 cells², and is ideally suited to the Kunkel directed mutagenesis method. This method relies on the cells producing plasmid DNA containing some uracil residues. The dut- and ung- mutations prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. Note that these cells contain an F’ which confers resistance to Chloramphenicol at low levels.

As Electrocompetent cells, the transformation efficiency is 1 × 10⁹ cfu/µg.

Functional genotype:
[F’ Tra+ Pil+ (CamR)] ung-1 relA1 dut-1 thi-1 spoT1 mcrA

² Joyce, C.M., N. Grindley 1984. Method for determining whether a gene of Escherichia coli is essential: application to the polA gene. J.Bacteriol. 158: 636-643

Genotype:
[F’ Tra+ Pil+ (CamR)] ung-1 relA1 dut-1 thi-1 spoT1 mcrA

MA136-CJ236-ElectroCompetent-Cells.pdf (194,713kb)

MSDS.pdf (118,131kb)