Phage Display Competent Cells
CAT# PRODUCT SIZE STORAGE 소비자가 주문
60522-1ER2738 Electrocompetent Cells12 rxns (DUOs)-로그인
60522-2ER2738 Electrocompetent Cells24 rxns (DUOs)-로그인
60512-1SS320 (MC1061 F') Electrocompetent Cells12 rxns (DUOs)-로그인
60512-2SS320 (MC1061 F') Electrocompetent Cells24rxns (DUOs)-로그인
60502-1TG1 Electrocompetent Cells12 rxns (DUOs)-로그인
60502-2TG1 Electrocompetent Cells24 rxns (DUOs)-로그인
60514-1MC1061 F- Electrocompetent Cells12 rxns (DUOs)-로그인
60514-2MC1061 F- Electrocompetent Cells24 rxns (DUOs)-로그인

Phage Display Competent Cells

  • Perfect for antibody phage display or peptide phage display library creation
  • The highest efficiency TG1 competent cells available: ≥4 × 1010 cfu/µg.
  • Your only source for electrocompetent SS320 and ER2738 cells
  • Amber suppressor and non-amber suppressor lines for maximum flexibility
  • High efficiency MC1061 F- cells for cloning or control reactions
  • Create larger libraries; speed discovery
  • Bulk custom dispensing available with all of our phage display cells
  • TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.
  • SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
    MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.  This strain not included in the sample kit.
  • ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.
Phage-Display-Competent-Cells-Transformation-Efficiency.jpg
Figure 1. Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification.
 
Table 1. Competent Cells for Phage Display libraries
Cell Lines
Transformation Efficiency
(cfu/µg pUC DNA)
Cloning Methylated DNA
BAC, Cosmid Cloning
Blue/White Screening
TG1 Electrocompetent
>4 × 1010
YES
NO
YES
IPTG induction required
SS320 (MC1061 F') Electrocompetent
>4× 1010
YES
NO
YES
IPTG induction required
MC1061 F- Electrocompetent
>4× 1010
YES
NO
YES
IPTG induction required
ER2738 Electrocompetent
>2 × 1010
YES
NO
YES
IPTG induction required
 
 
Genotype
  • TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
  • SS320 (MC1061F'): [F'proAB+lacIqlacZΔM15 Tn10 (tetr)] hsdR mcrB araD139 Δ(araABC-leu)7679ΔlacX74 galUgalK rpsL thi
  • ER2738: [F'proA+B+ lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
  • MC1061 F-: araD139 Δ(araA-leu)7697 Δ(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150 (StrR) spoT1 mcrB1 hsdR2
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