Phage Display Competent Cells
- Perfect for antibody phage display or peptide phage display library creation
- The highest efficiency TG1 competent cells available: ≥4 × 1010 cfu/µg.
- Your only source for electrocompetent SS320 and ER2738 cells
- Amber suppressor and non-amber suppressor lines for maximum flexibility
- High efficiency MC1061 F- cells for cloning or control reactions
- Create larger libraries; speed discovery
- Bulk custom dispensing available with all of our phage display cells
- TG1 Electrocompetent Cells. An amber suppressor strain (supE) prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.
- SS320 Electrocompetent Cells. A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.
MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010 cfu/µg) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage. This strain not included in the sample kit.
- ER2738 Electrocompetent Cells. An amber suppressor strain (glnV) prepared as highly efficient (≥2 × 1010 cfu/µg) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.™ Phage Display Kits.
Figure 1. Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
| Cell Lines |
Transformation Efficiency
(cfu/µg pUC DNA) |
Cloning Methylated DNA |
BAC, Cosmid Cloning |
Blue/White Screening |
|
TG1 Electrocompetent |
>4 × 1010 |
YES |
NO |
YES
IPTG induction required |
|
SS320 (MC1061 F') Electrocompetent |
>4× 1010 |
YES |
NO |
YES
IPTG induction required |
|
MC1061 F- Electrocompetent |
>4× 1010 |
YES |
NO |
YES
IPTG induction required |
|
ER2738 Electrocompetent |
>2 × 1010 |
YES |
NO |
YES
IPTG induction required |
IDT