PolyMAC
Due to low stoichiometry of phosphorylation, phosphopeptide enrichment is a critical step for successful phosphoproteomics experiments. The products described here, termed PolyMAC (Polymer-based Metal ion Affinity Capture), overcome the shortcomings of current enrichment methods that typically lack required reproducibility, phosphopeptide recovery and selectivity.
In this method, a soluble nanopolymer is functionalized with metal ions to specifically capture O-phosphorylated peptides. PolyMAC-Ti offers an efficient and greatly improved method to achieve more complete phosphopeptide enrichment under homogeneous conditions.
Performance Comparison
100ug of digested complex cell lysate from DG-75 B-cell lymphoma cells was used to compare the enrichment efficiency between titanium-based PolyMAC and other commercially available sources of phosphopeptide enrichment reagents. A number of unique phosphosites identified and percent enrichment selectivity were used as the parameters for comparison. Each experiment was run in triplicate.
IDT
