CAT#	PRODUCT	SIZE	STORAGE	소비자가	주문
30085-1	T4 DNA Polymerase, Exonuclease Minus	300 U		-	로그인
30085-3	T4 DNA Polymerase, Exonuclease Minus	1500 U		-	로그인

T4 DNA Polymerase, Exonuclease Minus

Only source for recombinant T4 DNA Polymerase, Exonuclease Minus.
Potential applications include enhancing DNA sequencing reads.

T4 DNA Polymerase, Exonuclease Minus is a DNA-dependent DNA polymerase which catalyzes the 5’→3’ synthesis of DNA and requires template and primer. Additionally, the enzyme lacks 3’→5’ exonuclease activity.

Source:
Purified from a strain of E. coli that carries a T4 DNA Polymerase, Exonuclease Minus expression plasmid.

Applications:

DNA Sequencing reaction.(1)

Heat inactivation: 70°C for 15 minutes.

Storage:
Enzyme and buffer should be stored at -20°C. Enzyme is stable for 12 months if handled properly.

Additional Information:

May increase the DNA sequencing read-lengths.

References

Nancy G. Nossal (1998) Genetics 148: 1535-1538.

MA070-T4-DNA-Polymerase-exo-minus.pdf (104,121kb)

MSDS.pdf (118,131kb)