CAT#	PRODUCT	SIZE	소비자가	주문
30241-1	NxGen™ T4 DNA Ligase (Low Concentration)	100,000 U	-	로그인
30241-2	NxGen™ T4 DNA Ligase (Low Concentration)	500,000 U	-	로그인
30243-1	NxGen™ T4 DNA Ligase (High Concentration)	150 rxns	-	로그인
30243-2	NxGen™ T4 DNA Ligase (High Concentration)	750 rxns	-	로그인

NxGen® T4 DNA Ligase

Reliably join double-stranded nucleic acid chains.

T4 DNA Ligase catalyzes joins strands of dsRNA and dsDNA by forming phosphodiester bonds. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

T4 DNA Ligase Version	Size	[Ligase]	Provided Buffer
Ligase Low Concentration	100,000 U	120,000 U/mL	10X T4 DNA Ligase Buffer
High Concentration Rapid Kit	150 rxn	600,000 U/mL	2X Rapid Ligation Buffer, 10X T4 DNA Ligase Buffer

10X T4 DNA Ligase Buffer is composed of 500mM Tris-HCI, 100 mM MgCl₂, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25°C. 2X Rapid Ligation Buffer is composed of 132mM Tris-HCI, 20 mM MgCl₂, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25°C.

Specifications

Unit Definition: 1 unit is defined as the amount of T4 DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1 X T4 DNA Ligase Buffer following a 30 minute incubation at 23°C.

Source: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene.

SS Exonuclease	6,000 U <0.1% released
DS Exonuclease	6,000 U <0.1% released
Endonuclease	6,000 U <0.1% converted
E. coli 16S rDNA Contamination	3,000 U <10 copies
Storage	-20°C

*Converted into equivalent units.

MA119-T4-Lig-LC.pdf (113,648kb)

MA120-T4-Lig-HC.pdf (114,980kb)

MSDS.pdf (118,131kb)